Week 2 Highlighted Parts

Question 1

Destruction of all forms of life including bacterial spore

Answer 1

Sterilization

Question 2

Destruction of some forms of life except bacterial spores

Answer 2

Disinfection

Question 3

Applied to skin; cannot be used as disinfectants

Answer 3

Antiseptic

Question 4

Applied to inanimate objects

Answer 4

Disinfectant

Question 5

Type of Organisms corresponds to?

Answer 5

Biochemical composition and mechanisms to protect themselves

Question 6

Microbial load corresponds to?

Answer 6

Total number of organism

Question 7

Fill in the blank; The _____ numbers of organism, the _____ the exposure time

Answer 7

higher; longer

Question 8

True or False? Organic material such as blood, mucus, and pus affects killing activity by inactivating the disinfecting agent

Answer 8

TRUE

Question 9

Organic materials affects killing activity by inactivating the disinfecting agent

Answer 9

Blood, mucus, and pus

Question 10

Most common methods of heat disinfection and sterilization

Answer 10

Autoclave

Question 11

Fill in the blank; steam under ___ atm of pressure, or ___ psi, ___ C for ___ minutes

Answer 11

1 atm, 15 psi, 121 degree celcius, 15 minutes

Question 12

Sterilization method for heat-stable substances that are not penetrated by moist heat

Answer 12

Dry Heat

Question 13

Sterilization method of choice for heat-stable object?

Answer 13

Heat

Question 14

Sterilization method of choice for glass wares

Answer 14

Dry Heat

Question 15

Fill in the blank; For pasteurization to occur, the heat should be at ___

Answer 15

63 degree celsius or 72 degree celsius

Question 16

Boiling occurs at what temp.?

Answer 16

100 degree celsius

Question 17

Requires thin membrane filters composed of plastic polymers or cellulose esters containing pores

Answer 17

Liquid filtration

Question 18

Pore sizes needed for liquid filtration of bacteria, yeasts, and molds

Answer 18

0.45 and 0.8 micrometer

Question 19

Pore size for critical sterilizing

Answer 19

0.22 micrometer

Question 20

Pore size for small viruses

Answer 20

0.01 micrometer

Question 21

Sterilization method for heat-sensitive solutions

Answer 21

Liquid filtration

Question 22

Method of filtration that requires HEPA filters

Answer 22

Air filtration

Question 23

What does “HEPA” stands for in HEPA filters

Answer 23

“high-efficiency particulate air” filter

Question 24

True or False? Air filtration removes microorganisms larger than 0.3 micrometer

Answer 24

TRUE

Question 25

Type of filtration used in laboratory hoods and in air systems

Answer 25

Air filtration

Question 26

Fill in the blank; Disinfection/Sterilization using Radiation (Ionizing) uses ___

Answer 26

Gamma rays or electron beam

Question 27

Fill in the blank; Disinfection/Sterilization using Radiation (Non-ionizing) uses ___

Answer 27

UV rays

Question 28

True or False? Gamma rays or electric beam has shorter wavelegth hence higher energy

Answer 28

TRUE

Question 29

True or False? UV rays has longer wavelegth hence lower energy

Answer 29

TRUE

Question 30

Sterilization method for disposable supplies

Answer 30

Ionizing radiation

Question 31

Disinfection method for surfaces

Answer 31

Non-ionizing radiation

Question 32

Chemical agents that may be used to sterilize

Answer 32

Chemosterilizers

Question 33

Fill in the blank; ___ percent ethyl or isopropyl alcohol may be used to disinfect or sterilized

Answer 33

60-90

Question 34

Component used in formaldehyde for disinfection and sterilization

Answer 34

Formalin

Question 35

Disinfection method for biosafety cabinets

Answer 35

Formaldehyde

Question 36

Disinfectant or Sterilant? 2% solution of glutaraldehyde for 10mins

Answer 36

Disinfectant

Question 37

Disinfectant or Sterilant? 2% solution of glutaraldehyde for 3-10hrs

Answer 37

Sterilant

Question 38

Sterilization method for heat labile medical equipment and materials that cant be sterilized with gas

Answer 38

Glutaraldehyde

Question 39

True or False? Aldehydes are active at alkaline

Answer 39

TRUE

Question 40

Iodine (5-10%) + neutral polymer =

Answer 40

Can be used as antiseptic (Iodophor)

Question 41

Most common iodophor

Answer 41

Polyvidone or povidone

Question 42

Contact time for Halogens (Iodophors)

Answer 42

> 30 seconds

Question 43

Two forms of hypochlorite (chlorine and chlorine compounds)

Answer 43

liquid sodium hypochlorite and solid calcium hypochlorit

Question 44

0.5% to 1% sodium hypochlorite =

Answer 44

Can be used as disinfectant

Question 45

Contact time for Chlorine and Chlorine Compounds (Hypochlorite)

Answer 45

Atleast 3 mins

Question 46

Disinfection method for water

Answer 46

Chlorine and Chlorine Compounds (Hypochlorite)

Question 47

Disinfection method for noncritical surfaces (benchtops and floors)

Answer 47

Detergents (Quaternary Ammonium Compound)

Question 48

Disinfection method for surfaces in hospitals and household environments

Answer 48

Phenolics

Question 49

Disinfection method for hands of surgical personnel and body of patients undergoing surgery

Answer 49

Chlorhexidine Gluconate (0.5% to 4%)

Question 50

Disinfection method for health care personnel handwash and also acts as surgical hand scrub

Answer 50

Chloroxylenol (O.5% to 4%)

Question 51

Chemical used for disinfection and sterilization that is commonly found in consumer and proffesional health care products

Answer 51

Triclosan

Question 52

Rarely used in clinical application and is slowly bactericcidal

Answer 52

Heavy metals

Question 53

Fill in the blanks; ___ of ethylene oxide per liter of chamber space at ___ for ___

Answer 53

450-700 mg; 55-60° C; 2 hours

Question 54

Sterilization method for materials that cannot withstand steam sterilization

Answer 54

Ethylene Oxide

Question 55

Type of containment barrier that protects the worker from the aerosolized transmission of organisms

Answer 55

Biological Safety Cabinets (BCS)

Question 56

True or false? Any procedure that has the ability to create aerosols should be performed in a BSC

Answer 56

TRUE

Question 57

What level of biosafety does it corresponds to? Minimal threat to laboratory personnel and environment

Answer 57

Biosafety level 1

Question 58

What level of biosafety does it corresponds to? Bacillus subtilis

Answer 58

Biosafety level 1

Question 59

What level of biosafety does it corresponds to? Agents that pose a moderate potential hazard for the employees and environment

Answer 59

Biosafety level 2

Question 60

What level of biosafety does it coresponds to? HBV, HIV, Salmonella spp., and Toxoplasma spp.

Answer 60

Biosafety level 2

Question 61

What level of biosafety does it coresponds to? Potential for aerosol transmission

Answer 61

Biosafety level 3

Question 62

What level of biosafety does it corresponds to? Diseases with these agents may have serious lethal

Answer 62

Biosafety level 3

Question 63

What level of biosafety does it corresponds to? M. tuberculosis, St. Louis encephalitis virus

Answer 63

Biosafety level 3

Question 64

What level of biosafety does it corresponds to? High risk of causing life-threatening infections, can be transmitted by aerosols, or have an unknown risk of transmission

Answer 64

Biosafety level 4

Question 65

What level of biosafety does it corresponds to? Marburg and Congo-Crimean; Hemorrhagic fever

Answer 65

Biosafety level 4

Question 66

Fill in the blank? Phenol Coefficient Test is also known as ___

Answer 66

Disinfectant test

Question 67

Fill in the blank? Phenol Coefficient Test utilizes ___ or ___ for the test

Answer 67

Staphylococcus aureus; Salmonella typhi

Question 68

Fill in the blank? Based on the dilution of phenol and experimental disinfectant are inoculated with the bacteria and incubated at ___.

Answer 68

20-37 deg C for 2-3 days

Question 69

True or false? Considering the Bartlett's Grading System, Scores of 0 or less indicate lack of iinflammation or presence of saliva

Answer 69

TRUE