Week 1 - Lecture 2b - Cellular Adaptation and Response to Stress, Injury and Death Flashcards
name three causes of cell stress and injury
deficiency
intoxication
trauma
Define deficiency
lack of substance necessary to the cell
- variety of specific chemicals
- energy supply
What can cause deficiency
nutritional deficiency
genetic disease
infection
Define intoxication
the presence of a substance that interferes with cell function
Origin intoxications
outside the cell - external : exogenous : biological or non-biological
Inside of the cell - internal : endogenous : biological
examples of external biological intoxication
produced by agents of infection
- bacteria, fungi
examples of external non-biological intoxication
injurious chemicals
- inhalation, swallowing
- need to achieve a dose high enough to cause injury
- therapeutic drugs - effective at prescribed level. can be fatal at high doses (barbiturates)
endogenous intoxication
genetic defect
abnormal accumulation
Define trauma
physical injury or damage resulting in loss of the cell’s structural integrity
some cases instant loss as a result of great damage
some cases physical damage arises gradually
Mechanisms of cell injury
direct (for example thermal injury) indirect : 3 major mechanisms - hypoxic cell injury -free radical injury - impaired calcium homeostasis
define hypoxia
cellular deprivation of oxygen
oxygen supply via blood is compromised
hypoxic injury
1 : result of ischemia : interruption of blood supply;to tissue
- rapid interruption is severe, metabolic needs are high
- angina pectoris, stroke, cerebral ischemia, transient ischemic attack (TIA)
- result of other conditions
-poor oxygen levels (anemia)
-imparied oxygenation (lung disease)
Toxic agents (CO, H2S, cyanide)
define anoxia
complete lack of oxygen in blood (absolute deprivation
8 steps for an ischemic injury
- blood flow compromised
- cell switch to anaerobic metabolism to maintain ATP level
- By-product is lactic acid (LA)
- Accumulating LA disrupts mitochondria
- decreasing ATP production results in dysfunction of membrane pumps
- Excessive sodium influx (Na/K ATP pump failed)
- Increased osmotic pressure (sodium is followed by water)
- Cell swell, waste products continue to accumulate
- 1. if hypoxia ceased : cell will not die
- 2. if not : cell dies
Re-perfusion injuries
‘secondary injury’
- re-perfusion of the tissue leads to further damage
- membrane pumps are still impaired
Free radicals and reactive oxygen species (ROS)
electrically uncharged atom or group of atoms having an unpaired electron that cause damage via :
:lipid peroxidation
Impaired calcium homeostasis
Ca2+ is an important second messenger and cytosolic signal for many cell responses
Intracellular Ca2+ concentration is low
– some Ca2+ is stored
Calcium activates enzymes in the cell
if Ca2+ homeostasis impaired
- intracellular Ca2+ increases
- mitochondria damaged, reduced ATP production
- intracellular destructive enzymes are activated
- cell membrane and cytoskeleton destroyed
Cellular adaptation and response to stress and injury
- cells have a variety of mechanisms to cope with stress
2. they can live through unfavourable conditions