VT 117 Flashcards
What involves pathogenic organisms?
Bacteriology
Bacteriology
Involves pathogenic organisms
Immunohematology
Blood bank
What involves the blood bank?
Immunohematology
Serology
Used to detect disease through antigen/antibody reactions
Used to detect disease through antigen/antibody reactions
Serology
Histology
Involves exam of tissues
Involves exam of tissues
Histology
Hematology
Includes cell count, hemoglobin, hematocrit, and coagulation
Includes cell count, hemoglobin, hematocrit, and coagulation
Hematology
Microbiology
Study of microscopic organisms
Study of microscopic organisms
Microbiology
What are characteristics of an in-house lab?
Good lighting/ventilation, washable surfaces only, low traffic area with storage, refrigerator/outlets/equipment, safety equipment, biohazard and sharps containers
What are some safety devices used in a lab?
Eyewash station, fire extinguisher, fire blanket, hoods
Safety concerns relating to dress/attire
Sturdy shoes, lab coats and scrubs, control hair, trim nails, minimal jewelry, dispose of sharps objects appropriately and immediately, wear gloves w/samples and chemicals
Accuracy
how closely results agree with the true quantitative value of the constituent
how closely results agree with the true quantitative value of the constituent.
Accuracy
Precision
the magnitude of random errors and the reproducibility of measurements
the magnitude of random errors and the reproducibility of measurements
Precision
Reliability
the ability of a method to be accurate and precise
the ability of a method to be accurate and precise
Reliability
Quality Control
procedures established to ensure that clinical testing is performed in compliance with accepted standards and that the processes and results are properly documented
Accuracy, precision, reliability; equipment maintenance; pre-, post-, analytic variables
Why is quality control important in a lab?
It keeps diagnostic reliability - so you can be sure that you are getting accurate results and giving a correct reading so the animal can be properly treated
What to do if you get a corrosive substance in your eye?
Rinse it with the eye wash for 15 minutes
pH levels
< 7 acidic
= 7 neutral
> 7 alkaline
What does the pH measuring?
The hydrogen ion concentration in a solution
What is meniscus?
a curve in the surface of a liquid when it touches another material
What is supernatant?
The liquid portion at the top of sediment after the solution has been centrifuged
The liquid portion at the top of sediment after the solution has been centrifuged
Supernatant
a curve in the surface of a liquid when it touches another material
Meniscus
Refractometer
Total Solids Meter, measures the refractive index of a solution to determine the specific gravity (urine and other fluids) or the protein concentration (plasma and other fluids)
Factors for autoclaving
appropriate PPE, appropriate packaging of the materials, appropriate materials, be careful of steam when opened and let cool
Centrifuge - what is and does
used to separate substances of different densities that are in a solution. The centrifuge spins samples at high speeds, which pushes the heaviest components in the sample to the bottom of the tube according to their densities. Liquid components are layered above the solid components, also according to their densities. When solid and liquid components are present in the sample, the liquid portion is referred to as the supernatant, and the solid component is referred to as the sediment
Metric unit
Kilo, hecto, deca, Basic Unit, deci, centi, milli
k h da L/g/m d c m
1000. 100. 10. 1. 0.1 0.01 0.001