Viral Vectors Flashcards
1
Q
What are viral vectors ?
A
viruses that are reprogrammed to express/repress a gene
2
Q
Uses of viral vectors ?
A
gene therapy
understanding brain function
disease modelling
3
Q
Types of viral vectors
A
lentivirus - ssRNA
Adenovirus - dsDNA
Adeno-associated virus - ssDNA
Herpes simplex virus - dsDNA
4
Q
Ideal viral vector
A
safe
long-term gene expression
efficient transduction, easy to produce
Ability to transduce proliferative and quiescent cells
cell specific transduction
5
Q
Expression casette
A
promotor
reporter e.g GFP, mCherry
gene of interest
