Transgenic Protocol Flashcards
Peptidergic nociceptors
Express trkA, CGRP, Sub P
projection terminate lamina 1 and outer lamina 2
non-myelinated, thinly myelinated
Non-peptidergic nociceptors
Express RET GDNF neurotrophic factor receptor
non-myelinated
projections ineer lamina 2
Express Mrgpr GPCR and bind the lectin IB4
when do the nociceptive neuron subgroups diverge from the common nociceptive group ?
late embryonic/ ealry postnatal E15
What happens at E15?
50% of neurons start to express RET and stop expressing trkA
They project too inner lamina 2 of dorsal horn
become neurotrophic factor independent
Express IBA & Mrgpr receptors
What are the main features of the common nociceptive group >
they all express trkA
dont have defined projections
Need NGF fro growth
Express negligible amounts of CGRP, SUB P, MRGPR,IB4
Global deletion of RUNX1 in mice ….
die due to defects in hematopoiesis E11.5
Rescue of RUNX1 KO by targeting expression to the hematopoietic cells
Survive until E19/birth
RUNX1 normally suppresses ?
peptidergic neurons
LOX P sites
specific DNA sequence recognised by Cre recombinase
GATA1 Promotor
Wnt1 Promotor
islet1 Promotor
liver
peripheral NS
neurons
Peptidergic genes that have increased expression in mice lacking RUNX1
CGRP
SUB P
Mu opoid receptor
DRASIC (acid sensing ion channel)
Non-peptidergic genes that have decreased expression in mic lacking RUNX1
Mrgprb4 Mrgprb5 Mrgprd MrgprA3 MrgprA4 MrgprA6 MrgprC11
NAV 1.9
P2X3
Von Frey test
Testing response to non-noxious stimulus
probing of hindpaw footpad with blunt probes records minimal probe thickness that evokes hind paw withdrawal
Pin Prick test
Testing for response to noxious stimulus
records the time it takes for
a mouse to withdraw its paw
when the paw is gently
probed with a sharp object
Over expression of RUNX1 causes suppression of ?
CGRP E17.5
