Microdialysis

Question 1

What is microdalysis used for

Answer 1

to allow monitoring of dynamic changes in extracellular conc of both exogenous and endogenous substances in the brain over a period of time in freely moving animals

Question 2

What compounds can be studied?

Answer 2

neurotransmitters
amino acids
hormones
drugs

Question 3

Components of micro-dialysis system

Answer 3

PE-10 tube - perfusate
semi-permeable membrane
fused sliica - dialyate
pump - .5-2ul / min

Question 4

Pore size of semi-permeable membrane

Answer 4

6-100kDa

Question 5

Probe implantation

Answer 5

direct favoured

indirect - cannula

Question 6

Histological Verification

Answer 6

infusion dye- fast green dye

histological stain - cresyl violet

Question 7

Outlet dead volume

Answer 7

volume of fluid in the tubing between the animal and the collection vial

Question 8

Range of analytes that can be measured is determined by?

Answer 8

the sensitivity of the analyte detection system
the MW of analytes
the volume collected
lipophilicity

Question 9

What does HPLC, ED and RIA stand for?

Answer 9

high performance liquid chromotography
Electrochemical detection
Radioimmunoassay

Question 10

List the HPLC methods that can be used

Answer 10

HPLC/ED
HPLC/fluorescence detection FD
HPLC/ optical absorption detection inultraviolet rangeUV
HPLC/MS
gas chromotraphy/MS
RIA
ELISA

Question 11

Neuropeptides and hormones measured by ?

Answer 11

RIA and ELISA

Question 12

Monoamine NTs measured by?

Answer 12

reverse-phase HPLC/ED

Question 13

Amino acids measured by?

Answer 13

ED, flourescent or UV detectors

- requires derivatization of amino acids on a precolumn

Question 14

Reverse-phase HPLC

Answer 14

Reversed-phase chromatography employs a polar (aqueous) mobile phase. As a result, hydrophobic molecules in the polar mobile phase tend to adsorb to the hydrophobic stationary phase, and hydrophilic molecules in the mobile phase will pass through the column and are eluted first.

Question 15

HPLC

Answer 15

is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Each component in the sample interacts slightly differently with the adsorbent material, causing different flow rates for the different components and leading to the separation of the components as they flow out the column.

Question 16

ACH measured using?

Answer 16

precolumn enzyme reaction to decompose acetylcholine to H2O2 which can then
be detected by reverse-phase HPLC/ED