Venipuncture/Phlebotomy Flashcards
What are the indications for phlebotomy?
venous blood sampling
What are the contraindications for phlebotomy?
- Cellulitis
- Trauma
- Burns
- Radical mastectomy
- AV fistula
- Hematoma
- Arm with IV line
What are the potential complications of phlebotomy?
- Skin infection (cellulitis)
- Infection of the vein (phlebitis)
- Thrombosis
- Laceration of the vein
- Accidental arterial puncture
- Hemorrhage or hematoma at site of puncture
- Vasovagal syncope
What equipment is needed for phlebotomy?
- Gloves and alcohol wipes, tourniquet
- 21 to 25 gauge straight needles or butterfly needles
- Vacutainer holder
- Alcohol wipes
- Bandaid oe 2x2 gauze and papertape
What is the phlebotomy procedure?
- Know what specific samples you need to collect and what order of draw for different tubes/lab tests
- Insure no latex allergies
- If failed first attempt, do not repeat at the same site
- Ask for help if needed
- Wash hands
- Explain procedure to pt and obtain consent
- Gather all equipment
- Have pt in supine position
- Use standard precautions
- Use upper extremity and have the pt extend the arm at the elbow
- Place tourniquet 2-4 inches above or 3 finger widths
- Not necessary to have the pt make a fist
- Cleanse the area with alcohol wipe. DO not touch the area after you clean it-if so, must reclean
- Apply gentle skin traction distal to the vein
- insert needle into vessel at a 30 degree angle and observe for flash of blood
- insert vacutainer tubes into vacutainer needle holder to collect sample
- Release tourniquet when last tube is 3/4 of the way full, remove tube from needle, and remove needle
- Apply pressure to the phlebotomy site with 2x2 gauze until bleeding stops
- apply bandage
What is a blue top tube used for?
- Coagulation studies, INR + KCCT, d-dimer, fibrinogen
What is a red top tube used for?
- bacteriology and viral serology
2. Selenium, zinc, 17 OHP, androgens, GH, vitamin D, INsulin, C peptide, antibiotic assays, DHAS, Cryoglobin, IGF1/GFBP3
What is a yellow top tube used for
Aldosterone, B12, ferritin, S folate, down’s screen, all routine biochemistry profiles except those mentioned above
What is a green top tube used for?
Carboxyhemoglobin, methemoglobin, cytogenetics
What are the lavender top tubes used for?
FBC, platelets, sickle cell test, malaria, HbA1c, Hb electrophoresis, CBC with diff
What are pink top tubes used for?
Blood group and cross matching
What are grey top tubes used for?
Blood glucose, ethanol, lactate
What are the the red/black tubes used for?
Combat, SST (serum separator tube) BMP
What is the order of draw for tubes?
- Blood cultures
- blue
- red
- yellow
- green
- Lavender
- Pink
- grey
Where are the most common locations for venipuncture?
- Medial cubital
- Cephalic
- Basilic vein