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Food Analysis > UV Vis Spectroscopy > Flashcards

UV Vis Spectroscopy Flashcards

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1
Q

Frequency Ranges

A

UV
Visable
IR
NMR

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2
Q

Spectroscopy

A

Production, measurement, interpretation of spectra from interaction of electromagnetic radiation with matter

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3
Q

Wave properties

A

Frequency
Wavelength
Amplitude

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4
Q

Dual nature of light

A

Acts as a wave and particle

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5
Q

Wavelength

A

Distance between two peaks or valleys on a wave

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6
Q

Wavenumber

A

Used for IR
1/wavelength

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7
Q

How does a spectrophotometer work?

A

Light source → light is separated out into different colors → filter → hits sample → detector

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8
Q

Absorption of radiation

A

Energy from a photon of electromagnetic radiation is transferred to sample

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9
Q

Absorption spectrum

A

Shows absorbance at different wavelengths

Take readings using wavelength of peak absorbance

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10
Q

Emission of radiation

A

Energy is released in the from of a photon of radiation

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11
Q

Fluorescence or phosphorescence

A

Emission of light by a substance that has absorbed an electromagnetic radiation

Absorption of electromagnetic radiation by a molecule → emit a photon of lower NRG

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12
Q

Applications of UV Vis

A

Randicity
Analyze minerals
Detect contaminants

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13
Q

UV wavelength range

A

200-350 nm

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14
Q

Vis wavelength range

A

350-700 nm

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15
Q

Transmittance

A

Ratio of light that enters and leaves the sample

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16
Q

Beer lambert law

A

Absorbance = absorptivity x path length x concentration
Only for dilute solutions

17
Q

UV sample prep

A

Want a clear, transparent, homogenous sample

18
Q

UV Vis: other things to keep in mind

A

Need a reference solution
Need the right cuvettes that don’t absorb the radiation

19
Q

Microplate readers

A

96 samples at a time!
Can do kinetics, microbial growth

20
Q

Chromogens

A

Chemicals that form colored compounds upon reaction with the compound of interest

React selectively
Form a soluble colored compound

21
Q

Quantifying minerals

A

Concentration of a mineral determined from a standard curve
Samples must be ashed or pretreated
Soluble mineral reacts with chromagen to produce a stable, colored product

22
Q

Colorimetric methods pros

A

Cheap
Simple
Accurate

Food Analysis (19 decks)

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