use of vectors Flashcards
what are vectors used for
transfering dna into a cell
what are the two types of vectors
invivo
invitro
define invivo
transfering the fragment of dna to a host cell using a vector
define invitro
using the polymerase chain reaction `
what is the vector for dna fragments
bacteria cells take in plasmid dna
what needs to happen inorder for bacteria to take in plamid dna
dna fragment needs to be inserted into plasmid
what are the two stages of insersion into a vector
- insersion into a plasmid
- isersion of plasmid into bacterium
describe and explain the process of insersion into a plasmid
- cut with the same restriction endonuclease
- complementry sticky ends
- dna fragment mixed with dna liagse
- dna anneals to plasmid
describe the proscess of insersion of plamisd into bacteria cell
- cool down bacteria - by placing into calcium chloride solution
- add plamids
- heat up
- encourages bacterium to take up plasmids
why do you cool down the bacterium cell
- to make bacteria walls more permiable to plasmids
the DNA on the nylon membrane is treated to form a single strand explain why
so that probes can anneal to DNA
what is a dna probe
a single strand of DNA
complementry to a specific gene
explain why genetc code inserted via plasmid from a lant is able to code for a protien in an insect
genetic code universal
insect dna can be transcibed
and can be translated
why might in a graph even thought the number of PCR cycles increases the number of DNA molecules remains constant
- dna free nucleotides have run out
what is the difference between the sense and anti- sense strands
sense strands code for production of a polypeptide
antisense strands are non-coding
