recombinant DNA technology Flashcards
1
Q
define biotechnology
A
any technology based on biology
2
Q
give examples of briotechnology
A
covid test
preganacy test
3
Q
how are dna fragments produced
A
recombination dna technology
4
Q
define recombinant DNA technology
A
recombining DNA from different organisms or from a single organism -‘transgenic’
5
Q
what are the two types of ways proteind can be made using recombinat dna technology
A
reverse transcriptase
restriction endonuclease
6
Q
describe the process of getting fragmented DNA using reverse transcriptase
A
- mRNA from specific gene e.g insulin gene
- mRNA is incubated with reverse transcriptase and free nucleotides
- free nucleotides complementry base pair with mRNA via reverse transcriptase
- cDNA formed
7
Q
A
8
Q
describe how you create recombinant DNA using restriction endonuclease
A
- restriction endonuclease cuts DNA at palandromic base sequence
- produces sticky ends
- promoter and terminator regions invloved
