pcr and reverse transcriptase

Question 1

what are the three main stages in the use of pcr

Answer 1

denaturation , annealing, dna synthasis

Question 2

what is the purpouse of pcr

Answer 2

used to amplify dna sample in vitro = allows losts of dna to be formed from a small sample

Question 3

what is a limitation of pcr

Answer 3

can only use a sample of dna max 1000

Question 4

define and explain the process first stage of pcr

Answer 4

denaturing where the template strand to be copied is seperated to a singe strand- this is done by placing the strand in a thermocycler and heated to 95 degrees inorder to destablise and break the hydrogen bonds forming a single strand

Question 5

define and explain the process in the second stage of pcr

Answer 5

annealing - cooled to 65 degrees
- primer added
- primers anneal to strands

Question 6

what is the third stage of pcr and explain the process

Answer 6

dna synthesis -
- solution heated to75 degrees
- dna polymerase binds complementory and free nucleotides

Question 7

how many strands of dna does one cycle of pcr produce

Answer 7

2

Question 8

what is the formular to work out no. of pcr produceed

Answer 8

n=2x ( where x is no. of cycles and n is no. of dna molecules

Question 9

what is the differance between a sense strand and an anti-sense strand.

Answer 9

sense strands gode for specific protiens
antisense strands are non coding