Urobilinogen/Bromsulfonthalein (BSP) BYE Extraction Test Flashcards
Colorless end product of bilirubin metabolism that is oxidized by intestinal flora to urobilin
Urobilinogen
T/F. Urobilin is also photosensitive
True
Urobilinogen Collection of sample:
Avoid light exposure
Urobilinogen Specimen:
2-hr freshly collected urine or freshly collected stool
Method use in Urobilinogen
Ehrlich’s Method
Ehrlich’s Method Reagent:
P.DABA (p-dimethylaminobenzaldehyde)
Urobilinogen Reference value:
1 Ehrlich = 1mg of urobilinogen
Urine:
0.1-1.0 Ehrlich unit/2hour or 0.54 Ehrlich unit/day urine
Stool:
75-275 Ehrlich unit/100g of feces
Complete Biliary Obstruction =
Decreased urobilinogen
T/F. Normally, B2 → intestine → urobilinogen
True
In complete biliary obstruction, B2 will not be oxidized to urobilinogen =
Decreased amount or absent urobilinogen
Bromsulfonthalein (BSP) BYE Extraction Test Principle:
addition of dyes/injection of dye (as waste) in the body
Test for hepatocellular function and potency of bile duct
Bromsulfonthalein (BSP) BYE Extraction Test
Rarely used because it utilize bromsulfonthalein dye which does not have any physiologic function since it is only a stain
Bromsulfonthalein (BSP) BYE Extraction Test
2 administration:
Rosenthal White Method
Mac Donald Method
Double collection method
Rosenthal White Method
Rosenthal White Method BSP Dose:
2mg/kg body weight
Rosenthal White Method Specimen collection: Blood (2)
o 5 mins after BSP administration
o 30 mins after BSP administration
Rosenthal White Method Reference value:
o After 5 minutes: 50% dye retention
o After 30 minutes: 0% dye retention
Single Collection method
Mac Donald Method
Mac Donald Method BSP Dose:
5mg/kg body weight
Mac Donald Method Specimen collection:
after 45 mins
Mac Donald Method Reference value:
After 45 minutes: +/- 5% dye retention (slightly more or less than 5%)
