ALBUMIN-PROTHROMBIN TEST Flashcards
- Part of the total protein and the most abundant type
- Concentration is directly proportional to the severity of hepatic disease
ALBUMIN
The liver synthesized _____ g/day of albumin
9-12
Low albumin is seen due to
decreased synthesis
ALBUMIN Reference value:
3.5-5.0 g/dL
Globulins are precipitated which removes globulin from total protein
Salt Precipitation
In Salt Precipitation, The remaining albumin from the total protein is quantitated using _______ _________
biuret reaction
Formula of Salt Precipitation
Total protein = Albumin + Globulin
T/F. In Salt Precipitation, We precipitate globulin to measure albumin
True
Charges protein positively so that the following anionic dyes would bind
Dye Binding
most commonly used but a sensitive dye to albumin
Bromcresol Green (BCG)
Most specific dye
Bromcresol purple (BCP)
Not specific for albumin
Methyl orange
more specific to albumin that methyl orange
2-4’-hydroxyazobenzene-benzoic acid (HABA)
Dye Binding Interferences:
conjugated bilirubin, aspirin, penicillin, sulfonamides
This confirm or check if the globulin is higher than albumin
ALBUMIN/GLOBULIN RATIO
seen in patient with cirrhosis, multiple myeloma, macroglobulinemia
Inverted A/G ratio
ALBUMIN/GLOBULIN RATIO Reference value:
Reference value: 1.3-3.1g/dL (CF to g/L: 10)
ALBUMIN/GLOBULIN RATIO Formula:
𝐴𝐺 𝑟𝑎𝑡𝑖𝑜 =𝐴𝑙𝑏𝑢𝑚𝑖𝑛
𝐺𝑙𝑜𝑏𝑢𝑙𝑖𝑛
Globulin = Total protein – Albumin
- Aka Vitamin K Response
- It differentiates intrahepatic disorder from extrahepatic disorder
PROTHROMBIN TEST
T/F. In PROTHROMBIN TEST, Administer vitamin K prior to the test intramuscularly (10 mg for up to 1-3 days) because there are factors that are Vitamin K dependent (Prothrombin Group)
True
PT Prolonged after vit. K administration =
Hepatic disorder
PT Normal after vit. K administration =
Extrahepatic disorder
prolonged prothrombin time signifies massive cellular damage
Acute/Chronic Hepatitis
