Urinalysis Flashcards
Define oliguria
Reduced urine production
Define isosthenuria
Urine of a concentration of 0
Define poikuria
Irregular passage of urine
Define Stranguria
Painful passage of urine
What is the function of the kidneys?
Filter out waste from the blood, regulate water in the body conserve proteins, electrolytes and other compounds that the body can use.
What is the normal volume of urine produced in cats and dogs?
1-2mls/kg/hr
Why is a urine sample beneficial?
Cheap, non-invasive, clients can do it themselves
A urine sample can direct the right way for more diagnostic tests. It can detect changes in other body systems . Can detect urinary dysfunction
What is the best case scenario for examining a urine sample?
Collected in the morning when the urine is most concentrated
It should be collected mid-flow to avoid contaminants
Ideally, the vulva or prepuce should be cleaned beforehand
It should be examined within 1 hour of collection
What equipment is needed for all types of urine sample
PPE
Clean collection tray/ tube or non-absorbant litter
Sterile sample pot - plain or boric acid (bacteriology) - labelled
Needle and syringe, clippers and skin scrub
Ultrasound machine
Catheter and sterile lube
What equipment is needed to place a urinary catheter?
- Urinary catheter
- Gloves
- Swabs and antiseptic
- Water-based lubricant
- Kidney dish to collect urine
- Speculum and pen torch (needed in females)
- sterile water, needle and syringe
- Urine collection bag ( and bung for closed bag)
- Suture material and tape
Describe the procedure of placing a urinary catheter
- Prepare equipment
- Get someone to restrain patient (sedation may be req)
- Wash hands and use PPE
- Clean area with antiseptic (may also need to clip)
- May need to use speculum and pen torch to see at this point
- Analgesic gel or lube with analgesic properties onto the catheter end
- Insert the catheter gently into the urethra (no touch method best where catheter comes straight out of bag and into urethra without being touched by gloves)
- Stop inserting when urine flows down the catheter (be careful not to go too far as may just be no urine in bladder)
- Fix in place if indwelling
What are some of the conditions that can cause urinary problems?
Renal disease/failure Dehydration Bladder rupture Diabetes mellitus Feline Lower Urinary Tract Disease
What are the advantages of collecting a free flow sample?
Non-traumatic Owners can do it at home Quite sterile in mid flow - esp cats Easy to do Good sample to do for haematuria tests as no iatrogenic element
What are the disadvantages of collecting a free flow sample?
Patient awkward
Urethral contamination
Contamination from collection vessels e.g. litter tray
What is manual expression urine sample?
Gently palpating the bladder - patient normally in lateral recumbency.
It should never be used in cases with urethral obstruction!
What are the advantages of manual expression?
Easy to perform
What are the disadvantages of manual expression?
Can cause bladder rupture/ trauma
Non-sterile
Uncomfortable for patient
Must be sufficient urine in bladder
What are the advantages of placing a urinary catheter?
Fairly sterile
Relatively easy to perform
Can introduce contrast media via catheter
Can be indwelling for monitoring urinary output
Helps avoid scald in recumbent patients
Can use to empty bladder pre-surgery
What are the disadvantages of placing a urinary catheter?
Cost Risk of iatrogenic haematuria/trauma Tricky in bitches May need sedation or GA Iatrogenic infection
What position for urinary catheter patient is a male dog, male cat and females in?
Male dog - lateral recumbency or standing
Male cat - dorsal or lateral recumbency
Females - sternal recumbency with legs hanging off table
What is a cystocentesis. Describe procedure
Where a sterile needle (20-22G 1-1.5inches) is passed through the abdominal wall into the bladder at a 45 degree angle , aspirate a sample from the bladder and then pressure is applied to stop the bleeding.
Clip and use surgical scrub - strict asepsis is needed!
What are the advantages of cystocentesis?
Relatively quick way to obtain a sterile sample
Patient generally tolerate well without GA
Sterile
Fairly painless
No urethral contamination
Iatrogenic infection uncommon
What are the disadvantages of cystocentesis?
Difficult technique - usually a VS
Contraindicative in extreme bladder distension
May require sedtion/ GA
Must be sufficient urine in the bladder for it to be effective
Can cause blood contamination of the sample
Risk of peritonitis (can cause leakage of bladder into the abdomen)
How long can urine be kept before examination?
1hr or 6hrs if refrigerated (warm to room temp before testing)
What is the concern when refrigerating crystals?
Refrigeration can cause formation of crystals which will interfere with microscope examination of the sediment
Urine should NEVER be frozen
Why is boric acid used in some containers?
The sample should be put into a boric acid container if its going for bacteriology.
The boric acid in the container prevents further growth of bacteria and preserves the existing bacteria
What is the normal odour of urine ?
Sour
What are the different smells of urine caused by?
Male cats have a much stronger smelling urine as they scent mark with pheromones)
Ketonic urine smells like pear drops (acetone smell) - can indicate diabetes mellitus
Some bacteria can change smell e.g. like ammonia-smelling
Foul smelling can indicate excess protein
What makes the urine more viscous?
An inflammation in the urinary tract will give rise to urinary muco-proteins which makes the urine more viscoid (sticky)
What colour is normal urine?
Pale yellow
What does clear urine indicate?
Dilute
Polydipsia/polyuria
What does dark yellow urine indicate?
More concentrated
Dehydrated
What does Green urine indicate?
If there is also foam on the surface it indicates biliverdin (oxidation of bilirubin)
What does brown/red urine indicate?
Haematuria or certain drugs can also cause this
What does blue/green urine indicate?
Some drugs alter the colour of urine as does some foods e.g. beetroot
Why does normal urine go cloudy on standing?
Because microorganisms decompose the urea to ammonia which increases the pH and causes precipitation of phosphate.
Why is rabbit urine cloudy?
Due to the presence of calcium carbonate crystals
Name some things that can affect the turbidity of urine
RBCs Crystals or casts Cells Yeast Fungi Pus Phosphate precipitation Prostate/vaginal secretions
What is the specific gravity?
The weight of a known volume of fluid compared to the same volume of distilled water (SG of 1.000). It is an estimation of the concentrating ability of the kidney
What are the normal SG values for dogs and cats?
Dog: 1.015-1.045
Cat: 1.020-1.040