Urina 4.1.2

Question 1

A slightly cloudy body fluid is submitted for a cell count. The technologist will make which of the following recommended WBC dilutions for slightly cloudy specimens before performing the cell count?
Undiluted
1:2
1:10
1:20

Answer 1

1:2

Question 2

All of the following diluents can be used for a WBC count on synovial fluid except

3% acetic acid.
hypotonic saline.
hyaluronidase buffer.
0.85% saline.

Answer 2

3% acetic acid.

Question 3

Viscous body fluids require the use of which type of pipette?
•Sterile transfer pipette
• Positive displacement pipette
• Air displacement pipette
• Serologic pipette

Answer 3

• Positive displacement pipette

Question 4

Semen that fails to liquefy after 60 minutes must be pretreated using which of the following before performing sperm counts, motility assessment, and chemical tests?
• Sodium bicarbonate/formalin mixture
• Bromelain
• Turk’s solution
• Warming in a 37 °C water bath for 30 minutes

Answer 4

• Bromelain

Question 5

Which of the following ensures an accurate cell count when using a hemacytometer?
1. Avoiding high dilutions on body fluids
2. Counting additional squares if the count is low
3. Adjusting calculations based on the volume of body fluid counted
4. Performing and analyzing dilutions in duplicate
• 1 and 2
• 1, 2, and 4
• 3 and 4
• All of the above

Answer 5

• All of the above

Question 6

What is the cell count on a slightly cloudy CS that was performed using a 1:2 dilution, counting the five large squares and giving duplicate counts of 45 cells for each count?
• 22.5 × 106 WBCs/L•
•45 × 106 WBCs/L
• 90 × 106 WBCs/L
• 180 × 10° WBCs/L

Answer 6

• 180 × 10° WBCs/L

Question 7

All of the following characteristics are true about the preparation of slides using centrifugation except
•preserves cellular morphology.
• easiest to perform.
• good cellular recovery.
•least expensive to use.

Answer 7

• good cellular recovery

Question 8

A nucleated cell count of 0 to 100 cells/uL requires how many drops of body fluid when preparing a slide by cytocentrifugation?
• 2
• 3 or 4
• 5 or 6
• 10

Answer 8

• 10

Question 9

All of the following cellular distortions are associated with cytocentrifugation except
• nuclear lobes of PMNs take on a pyknotic appearance.
• cells at the center of the cell button may be small and have denser nuclear chromatin.
• cytoplasmic vacuoles and/or granules localize along the cell periphery.
• irregular cytoplasmic processes form.

Answer 9

nuclear lobes of PMNs take on a pyknotic appearance.