Unwinding the DNA

Question 1

What proteins are involved in unwinding the DNA?

Answer 1

DNA helicases: unwinds the DNA and is assisted by topoisomerase
topoisomerases: prevent supercloiling
Single-stranded DNA binding proteins: keep DNA unwound

Question 2

what do single-stranded DNA binding proteins bind to?

Answer 2

single-stranded DNA at the replication fork, prevent re-annealing