Lagging Strand Synthesis Flashcards
1
Q
what is required for lagging strand synthesis?
A
primosome required: primase (an RNA polymerase)
and auxillary proteins
2
Q
what does primase do?
A
synthesizes short RNA rpimer
3
Q
why kind of replication is involved with lagging strand synthesis?
A
discontinuous replication, reads 3’ to 5’ template
4
Q
what type of polymerase is involved with lagging strand synthesis?
A
DNA polymerase III: synthesizes 5’ to 3’
5
Q
what does DNA polymerase III generate?
A
short okazaki fragments
1000 - 2000 bp in prokaryotes
100 bp in Eukaryotes
