Directionality of DNA synthesis Flashcards
1
Q
catalyzed by what?
A
DNA polymerase
using triosphosphate deoxynucleoside derivatives
2
Q
what are the four bases of triosphosphate deoxynucleoside derivatives
A
dATP
dTTP
dGTP
dCTP
3
Q
what do triosphosphate deoxynucleoside derivatives do?
A
cleavage of 2 terminal phosphates provides the free energy for polymerization
4
Q
where does DNA synthesis always occur?
A
3’ end covalent bond formed
5
Q
what bond also occurs during DNA synthesis
A
phosphodiester bond, between the 3; OH group of the terminal nucleotide. leaves 3’ end with free OH group
6
Q
which direction does synthesis proceed?
A
in the 5’ -> 3’ direction
