Unit Three Flashcards
What is cellular respiration
Process in which cells consume O2 and produce CO2
What are the three majors stages of cellular respiration
Acetyl CoA production, acetyl CoA oxidation, electron transfer and oxidative phosphorylation
Acetyl Co A production generates what
ATP, NADH, FADH2
Acetyl co A oxidation generates
NADH, FADH2, and one GTP
Oxidative phosphorylation generates a lot of
ATP
Glycolysis occurs where
Cytoplasm
Citric acid cycle occurs where
Mitochondrial matrix
What part of the citric acid cycle occurs in the inner membrane of mitochondria
Succinct enough dehydrogenase
Oxidative phosphorylation occurs where
Inner mitochondrial matrix
What is the net reaction of conversion of pyruvate to acetyl co a
Oxidative decarboxylation of pyruvate, first carbons of glucose to be fully oxidized
Site of conversion of pyruvate to acetyl Co-A
Mitochondrial matrix
What catalyze the conversion of pyruvate to acetyl Co A
Pyruvate dehydrogenase complex
What is the only reaction with C-C bond formation
Citrate synthase
What does citrate synthase do
Condensation of acetyl CoA and oxaloacetate
Citrate synthase uses what type of catalysis
Acid/Base
What is the good electrophile of oxaloacetate
Carbonyl group
What is not a good nucleophile of acetyl CoA
Methyl
What is the rate limiting step of CAC
Acetyl Co A to citrate using citrate synthase
Activity of citrate synthase depends on
Oxaloacetate
Citrate synthase goes through conformational change when it binds with
Oxaloacetate
Open conformation of citrate synthase does not have a binding site for
Acetyl CoA
Closed conformation for citrate synthase occurs when
Binding of OAA which creates binding site for acetyl Co A
Citrate to isocitrate is what type of reaction
Dehydration/rehydration
From citrate to cis-aconitate what occurs
Removal of water causing a cis C=C bond
Why go from citrate and cis-aconitate
Citrate is a tertiary alcohol and is a poor substrate for oxidation
Isocitrate is a secondary alcohol and is a good
Substrate for oxidation
What catalyze the water removal from citrate and subsequent addition to cis-aconitate
Iron sulfur center which sensitive to oxidative stress
