Unit C: Cycling of Matter In Living Systems | Outcome 1 | L 1 - L2 Flashcards
What are the 5 Basic Principles of Being Alive?
All living things must be able to:
1. Grow
2. Reproduce
3. Respond to stimuli
4. Use and require energy
5. Produce wastes
What is spontaneous generation also known as?
Abiogenesis
What are the three principles of Cell Theory?
- All living things are made up of one or more cells
- Cells are the smallest functional unit of life
- All cells are produced from preexisting cells through the process of cell division
How did Zacharias Janssen (and Hans Jassen) contribute to cell theory
Invented the first compound microscope (1590)
How did Robert Hooke contribute to Cell Theory
Observed compartments in cork (plant cells) and first named them cells. Led to the understand of cells as the smallest unit and of life
How did Antoine van Leeuwenhoek contribute to Cell Theory?
Using a better, single lens in a microscope that was made from higher quality glass, he was first to observe and describe single-celled organisms (he called them animalcules) among other cells.
How did Matthias Schleiden and Theodore Schwann contribute to Cell Theory?
Matthias Schleiden: Concluded all plants are composed of cells
Theodore Schwann: Concluded all animals are composed of cells
How did Rudolf Virchow contribute to Cell Theory?
- Observed cells dividing under a microscope
- Concluded that all cells divide to produce more cells
What two types of Electron Microscopes are there?
- Transmission Electron Microscope
- Scanning Electron Microscope
What is a Transmission Electromagnetic Microscope used for and how does it work?
- To study the internal structure of cells
- Electron beam passes through a thin section of tissue covered in plastic and strikes a fluorescent screen to produce a 2D image
What are Scanning Electron Microscopes Used For and how does it work?
- Used to view external shapes (3D image)
- Specimen is coated with a material that will reflect electrons and make them hit a sensor
Describe the function of:
Eye piece (Ocular lens)
Observe the specimen by looking through
Describe the function of:
Body tube
Supports the ocular lens
Describe the function of:
Arm
- Supports the revolving nosepiece & body tube.
- Used to carry the microscope.
Describe the function of:
Revolving nosepiece
Holds (supports) the objective lenses.
Describe the function of:
Objective lenses
Different levels of magnification power
(Low, medium, high, etc)
Describe the function of:
Stage
Is where the slide is placed for support and viewing
Describe the function of:
Stage clips
Holds the slide in place
Describe the function of:
Diaphragm
Regulates the amount of light passing through the stage opening.
Describe the function of:
Coarse adjustment knob
- Moves the stage up and down.
- Use only with low power.
Describe the function of:
Fine adjustment knob
- Makes the image sharper and clearer.
- Use with medium and low power
Describe the function of:
Light source
Supplies the light for viewing the specimen on the slide
Describe the function of:
Base
Supports the entire microscope and is also used when carrying the microscope.
What is the formula for calculating total magnification?
Total magnification = ocular lens x objective lens
What is FOV?
Regarding microscopes
The diameter of the “circle”
you can see through the
microscope
Increase magnification = FOV becomes smaller
How do you convert:
mm (milimeters) to μm (micrometers)
1 mm = 1000 μm
Multiply by 1000
How do you convert:
μm (micrometers) to mm (milimeters)
1000 μm = 1mm
Divide by 1000 (Multiply by 1mm/1000μm)
Describe the formula to calculate:
FOV on high or medium power
After measuring FOV on low power
M1D1 = M2D2
M1 = magnification on first (given) lens
D1 = Diameter of FOV under first (given) lens
M2 = magnification on second (asked for) lens
D2 = Diameter of FOV under second (asked for) lens
What are the rules for making a:
Biological Diagram
- Use plain, white paper
- Use PENCIL only
- Use half a page for each diagram
- Draw a cluster of a few cells (not all of them)
- Draw only what you see (eg. The cells are not all
perfect rectangles; there should not be any broken
lines) - Draw the outline of the structure only. NO SHADING
OR COLORING - Draw labels with a ruler, horizontal line and all labels
should line up on the right side of the diagram - At the bottom of the diagram: Figure 1: Description of
the drawing - Include the type of preparation (wet mount, dry
mount, prepared slide), name of specimen, type of
stain used or unstained, magnification