unit 9 (antimicrobial methods and eukaryotic microorganisms) Flashcards
two factors that influence the level of cleanliness required for a particular fomite
application for which the item will be used, level of resistance to antimicrobial treatment by potential pathogens
sterilization definition
the complete removal or killing of all vegetative cells, endospores, and viruses from the targeted item or environment
sterilization can be accomplished through
physical means: high heat, pressure, or filtration and chemical means
sterilants definition
chemicals that can be used to achieve sterilization
aseptic technique definition
involves a combination of protocols that collectively remain sterility, or asepsis, thus preventing contamination of the patient with microbes and infectious agents
antisepsis definition
reduces microbial load on skin or tissue through application of antimicrobial chemical
sepsis definition
a systemic inflammatory response to an infection that results in high fever, increased respiratory and heart rates, shock, and possibly death
sterile field definition
a designated area that is kept free of all vegetative microbes, endospores, and viruses
disinfection definition
reduces or destroys microbial load of an inanimate object through application of heat or antimicrobial chemicals
ideal characteristics for disinfectants/antimicrobials
fast acting, stable, easy to prepare, inexpensive, and easy to use
disinfection is not sterilization because
some microbes remain, endospores tend to survive even when all vegetative cells have been killed
what are antiseptics
chemicals safe for use on living skin or tissues
critical items definition
must be sterile because they will be used inside the body, often penetrating sterile tissues or the bloodstream
semi-critical items definition
items that do not need to be sterilized but require a high level of disinfection because they come in contact with mucous membranes or nonintact skin but do not penetrate tissues
noncritical items definition
items that need to be cleaned but not highly disinfected because they come in contact with, but to not penetrate, intact skin
degerming definition
microbial numbers are greatly reduced by gently scrubbing living tissue with a mild chemical
sanitization definition
cleansing of fomites to remove enough microbes to achieve levels deemed safe for public health.
-cide or -cidal refers to
physical and chemical methods of microbial control that kill the targeted microorganism
-stat or -static refers to
physical or chemical methods that do not kill the organism, but stop their growth
factors that determine whether a particular treatment is -static or -cidal
types of microorganisms targeted, the concentration of chemicals used, and the nature of the treatment applied
microbial death curve
how degree of microbial control is evaluated, describes the progress and effectiveness of a particular protocol
decimal reduction time (DRT) definition
the amount of time it takes for a specific protocol to produce a one-order-of-magnitude decrease in the number of organisms, or death to 90% of the population
factors that contribute to the effectiveness of an antimicrobial protocol
length of exposure, amount of microbes, susceptibility of microbe to antimicrobial, concentration of antimicrobial, temperature, conditions that limit the contact between the agent and the microbe
physical methods of control examples
heat, cold, pressure, desiccation, radiation, sonication, filtration
thermal death point (TDP) definition
the lowest temperature at which all microbes are killed in a 10 minutes exposure
thermal death time (TDT) definition
length of time needed to kill all microorganisms in a sample at a given temperature
boiling kills
kills vegetative cells and some viruses
boiling mode of action
moist-heat control that denatures proteins and alters membranes
categories of heating protocols
dry-heat sterilization and moist-heat sterilization
dry-heat sterilization examples
aseptic technique of inoculating loops, incineration
moist-heat sterilization example
autoclave
most effective heat sterilization category is __ because
moist-heat sterilization because it can penetrate cells better than dry heat does
autoclaves function by
raising temperatures above the boiling point of water (steam) and pressure to sterilize items
autoclaves kill
vegetative cells, viruses, and endospores (everything except for prions basically)
autoclave mode of action
moist-heat control that kills cells by denaturing proteins and alters membranes
autoclave tape function
indicates when appropriate temp is reached: white strips turn black when appropriate temp is achieved
biological indicator spore test function
uses strip of paper of endospores to determine whether endospores were killed or not during the autoclave process
Diack tube function
indicates when proper sterilization temperature is reached in an autoclave; a temperature sensitive pellet melts at proper sterilization temp
traditional pasteurization kills
pathogens and reduces the number of spoilage causing microbes
pasteurization mode of action
denatures proteins and alters membranes
high-temp short-time (HTST) pasteurization
exposes milk to 72 degrees for 15 secs, which lowers bacteria numbers while preserving quality
ultra-high-temp (UHT) pasteurization
milk is exposed to 138 degrees for 2 seconds, causing slight change to taste and smells
refrigeration mode of action
inhibits metabolism, slows or arrests cell division
freezing mode of action
stops metabolism, may kill microbes
high-pressure processing kills
vegetative bacteria (not endospores), yeast, molds, parasites, and viruses (not endospores)
high-pressure processing mode of action
denatures proteins and can cause cell lysis
hyperbaric oxygen treatment mode of action
inhibits metabolism and growth of anaerobic microbes
desiccation kills
can kill all types of cells because all cells require water, except endospores
simple desiccation (drying) mode of action
inhibits metabolism
reduce water activity (addition of solute) mode of action
inhibits metabolism and can cause cell lysis
lyophilization (freeze-drying) mode of action
inhibits metabolism
ionizing radiation types
x-rays, gamma rays, and high-energy electron beams
ionizing radiation mode of action
sterilizes by altering molecular structures, introduces double strand breaks into DNA
ionizing radiation is used on
packaged items that cannot be autoclaved (plastic)
what is nonionizing radiation
ultraviolet light that sterilizes but does not penetrate cells or packaging
nonionizing radiation mode of action
introduces thymine dimers, leading to mutation that eventually kill the cell
sonication definition
use of high frequency sound waves to disrupt cell structures
sonication mode of action
cavitation disrupts cells, lysing them
cavitation definition
formation of bubbles inside the cell
filtration mode of action
physically removes microbes from air or liquid
high efficiency particulate air (HEPA) filters trap
bacterial cells, endospores, viruses, nearly sterilizing the air
membrane filters remove __ from ___
microbes from liquid samples
factors to consider when choosing chemical antimicrobials
type of microbe targeted, how clean the item needs to be, the disinfectant’s effect on item integrity, its safety, its expense, and its ease of use
chemical antimicrobial examples
phenolics, heavy metals, halogens, alcohols, surfactants, bisbiguanides, alkylating agents, peroxygens, supercritical fluids, and chemical food preservatives
phenol mode of action
inhibit microbial growth by denaturing proteins and disrupting membranes
phenols are used in
mouthwash and toothpaste, lysol, agriculture, impregnated into products
phenolics characteristics
tend to be stable and persistent on surfaces
heavy metals mode of action
kill microbes by binding to proteins, thus inhibiting enzymatic activity
oligodynamic definition
very small concentrations show significant antimicrobial activity
heavy metals examples
mercury, silver, copper, nickel, zinc
mercury mode of action
bacteriostatic; bind to sulfur-containing amino acids within proteins, inhibiting their functions
silver is used as __ in __
antiseptic in antibiotic creams, making them thousands of times more effective
copper sulfate is a common
algicide used to control algal growth in pools and fish tanks
halogen examples
iodine, chlorine, and fluorine
iodine mode of action
disinfects by oxidizing cellular components
chlorine mode of action
disinfects by producing a strong oxidant that is uncharged and enters cells easily
chloramine characteristics
disinfect, stable, release chlorine over long periods of time
fluorine mode of action
accumulates in plaque-forming bacteria, interfering with their metabolism
alcohol mode of action
disinfect and antiseptic by rapidly denaturing proteins, inhibiting cell metabolism, disrupting membranes, leading to cell lysis
alcohols effective against
bacteria, fungi, enveloped viruses
surfactant definition
group of chemical compounds that lower the surface tension of water
surfactant mode of action
degerm by physically lifting away dirt and microbes from surfaces and skin
quaternary ammonium salts (quats) mode of action
insert into the bacterial phospholipid bilayer and disrupt membrane integrity
characteristic of quats
stable, nontoxic, inexpensive, colorless, odorless, and tasteless
quats kill
fungi, protozoans, bacteria, enveloped viruses (not endospores or nonenveloped)
bisbiguanide (chlorhexidine) mode of action
disrupts cell membranes and causes cell’s cytoplasmic contents to congeal and is bacteriostatic at lower concentration, bactericidal at higher
bisbiguanide (chlorhexidine) kills
yeasts, gram+ and - bacteria, enveloped viruses
alkylating agents mode of action
replace a hydrogen atom within a molecule with an alkyl group, thereby inactivating enzymes and nucleic acids
alkylating agents examples
formaldehyde, glutaraldehyde, o-phthalaldehyde (OPA), ethylene oxide, beta-propionolactone
formaldehyde kills
bacteria, viruses, fungi, and endospores
formaldehyde leads to
sterilization at low temperatures
ethylene oxide
highly penetrating, gaseous sterilizer
peroxygen examples
hydrogen peroxide, peracetic acid, benzoyl peroxide, carbamide peroxide, ozone gas
hydrogen peroxide mode of action
produces free radicals that damage cellular macromolecules
hydrogen peroxide kills
gram- and + bacteria (more effective against gram+), fungi, viruses, endospores, decreased efficacy against bacteria that produce catalase and peroxidase
peracetic acid characteristics
can be used as a liquid or plasma sterilant, readily kills endospores, more effective than hydrogen peroxide, immune to inactivation by catalase and peroxidase, and breaks down into environmentally innocuous compounds