Unit 5.1 Flashcards
Coenzyme: NAD
name the group transferred
electrons (hydride ion)
Coenzyme: FAD
name the group transferred
Electrons
Coenzyme: heme in cytochromes
name the group transferred
electrons
Coenzyme: Pyridoxal phosphate
name the group transferred
amino groups
Coenzyme: Coenzyme A
name the group transferred
Acyl groups
coenzyme: biotin, tetrahydrofolate, S-adenosylmethionine
name the group tranferred
One-carbon groups
name the 4 types of catalytic strategies
- general acid-base catalysis
- covalent catalysis
- metal ion catalysis
- proximity and orientation
turnover number
Kcat - the number of substrate molecules converted per enzyme molecule at saturation
specificity constant
Kcat/Km - measures catalytic efficiency (rxn of free enzyme w/ substrate to product)
Which of the following are inhibitors:
A. Lead
B. Statins
C. Aspirin
D. Penicillin
E. Ibuprofen
F. Methotrexate
All of them
What type of inhibitor are the following:
A. Lead
B. Statins
C. Aspirin
D. Penicillin
E. Ibuprofen
F. Methotrexate
A. non-competitive
B. competitive
C. irreversible
D. irreversible
E. competitive
F. methotrexate
Which of the following are major enzyme classes
A. Oxidoreductases
B. Transferases
C. Hydrolases
D. Lyases
E. Isomerases
F. Ligases
G. Proteases
A, B, C, D, E, F
What do oxidoreductases do?
move electrons between molecules
what do transferases do?
move chemical groups between molecules
what do hydrolases do?
break bonds with water (e.g proteases)
what do lyases do?
break bonds without water. add or remove atoms to or from a double bond
what do isomerases do?
change molecular shapes by transferring groups within molecules
what do ligases do?
create new bonds between molecules by condensation reactions
- ATP dependent
what are the advantages of enzyme associations?
- enzymes are associated with metabolic coupling. this increases efficiency by channeling products between enzymes
- different configurations:
1. enzyme aggregates
2. assembly on scaffolds
3. multi-domain multifunctional enzymes
