Unit 5 Chapters 20, 27-31 The Evolutionary History Of Biodiversity, Biotechnology, Plants, Fungi, Protists, Bacteria, And Archrea Flashcards
Which of the recombinant DNA drugs is incorrectly matched with its indication (the disease it is prescribed totreat)?
Neupogen (Amgen): Multiple sclerosis (Interferon)
Ian Wilmut
Cloning of Dolly the Sheep
Jennifer Doudna
CRISPR Cas9
W. French Anderson
First Human Gene Therapy
Shinya Yamanaka
induced Pluripotent Stem Cells
Stanley Cohen
Isolation of bacterial plasmids
Kary Mulis
Polymerase chain reaction
Herb Boyer
Isolation of restriction enzymes, Founder of Genentech
James Thompson
Derivation of Human embryonic stem cells
Fred Sanger
Dideoxy DNA sequencing
Craig venter
Whole genome shotgun sequencing
In the polymerase chain reaction (PCR) technique, a heating phase and a cooling phase alternate in cycles. Anoriginal sample of DNA would have to pass through how many total cycles to amplify the DNA ~1 billiontimes? (What about 2 billion times? What about 1 trillion times?)
C. 30
This figure shows the steps in cloning the human insulin gene. What process or enzyme was used for Step 1 ?
C. A restriction enzyme
Gene therapy treatments with new drugs like Kymriah and Yescarta (select all that apply)
B. The first FDA-approved drugs for immunotherapy to treat specific treatments
C. Involves the use of genetically engineered Chimeric Antigen Receptor T-cell to recognize proteins like CD19 on cancer cells
Match the drug with the disease ? Breast Cancer - HER 2+
Herceptin
Non-Hodgkin Lymphoma CD20+
Rituxin
T-cell autoimmune diseases like Rheumatoid arthritis and Psoriasis
Enbrel
RSV
Synagis
Cancer (antiVEGF, anti angiogenesis)
Avastin
PAM
A 3-6 nucleotide DNA sequence immediately downstream of the target site in the genomic DNA to be edited
Cas9
An ending lease that can create double-stranded breaks in DNA
SgRNA
An RNA molecule engineered to be complementary to the target to be edited
Which of these statements is NOT TRUE regarding the procedure for making cloned animals by somaticcell nuclear transfer?
The donor nucleus is from a haploid, quiescent cell
Idaho Gem
First cloned mule
Dolly
First cloned mammal
Prometia
First cloned animal that was born from its genetic twin
Zong Zong
First cloned macaque for neuroscience research
CC
First coined housecoat (female calico)
Joy
First cloned piglet engineered for human organ transplant
Human embryonic stem cells (hES cells) and hiPS may be useful in transplant medicine because the cells:
a. Are pleuripotent and can form virtually any cell type in the body with appropriate hormones and growthfactors
b. Make the enzyme telomerase, allowing them to replicate indefinitely in tissue culture
c. Have the same genetic karyotype as a normal human cell
d. Can be prepared to provide an exact tissue match to a patient
e. All of the above
All of the above
In 2007, it was reported that adult, differentiated human skin cells can be made to behave as pluripotentstem cells by the introduction of a few genes: Oct 4, Sox2, Kfl4 and c-myc. What concern has limited thepotential of human Induced Pleuripotent Stem Cells as human therapeutics?
The gene c-myc is an oncogene that causes cancer phenotypein about 25% of the cell cultures
Human embryonic stem cells (hES cells) derived from blastocysts donated after IVF (in vitro fertilization)have been successfully used in research to: (select all that apply)
a. Create new mesodermal cells like beating heart cells
b. Create new ectodermal cells like dopamine-producing nerve cells
c. Create new endodermal cells like insulin-secreting pancreatic islet cells
d. Create a cloned human from stem-cell derived eggs and sperm
a. Create new mesodermal cells like beating heart cells
b. Create new ectodermal cells like dopamine-producing nerve cells
c. Create new endodermal cells like insulin-secreting pancreatic islet cells
The bacteria Bacillus thurengiensis (Bt)
a. naturally makes a crystal protein toxic to Lepidopteran insects
b. causes food poisoning in people who eat the crystal protein
c. is the active ingredient in the insecticide Dipel
d. all of the above
e. a and c
A. Naturally naked a crystal protein toxic to Lepidopteran insects
C. Is the active ingredient in the insecticide Dipel
In the crop called Golden Rice, this genetically modified food
a. contains 4 genes from daffodils and bacteria that together produce the pigment B-carotene in rice
b. holds the potential for preventing the deaths of millions of people every year
c. can help prevent blindness resulting from vitamin A deficiency
d. will be offered at low cost or no cost to developing countries
e. all of the above
All of the above
Crops that are RoundUp Ready
a. Make the herbicide RoundUp in every cell of the plant
b. Kill weeds in the fields where they are growing
c. Are genetically modified to be resistant to treatment with RoundUp herbicide
d. Contain a mutant form of the enzyme EPSP synthase that is unaffected by RoundUp herbicide
e. c and d only
e. C and D only
A dideoxy ("chain terminating") nucleotide used in DNA sequencing differs from a deoxynucleotide as it has
a. An -OH at the 3-carbon and an -OH at the 2-carbon of deoxyribose
b. A "U" instead of an "A" in base-pairing
c. An -H at the 3-carbon and an -OH at the 2-carbon of deoxyribose
d. An -H at the 3-carbon and an -H at the 2-carbon of deoxyribose
e. Ribose as the sugar and 3 phosphates from ATP
D. An -H at the 3-carbon and an -H at the 2 carbon of deoxyribose
Some bacteria avoid a host's immune system by means of:
a. efficient use of their flagella.
b. cloaking the cell with host proteins.
c. cloaking the cell with a watery capsule.
d. changing their cell wall structure to resemble that of their host.
e. eliminating the use of a membrane
C. Clocking the cell with a watery capsule
Even though bacteria lack membrane-bound organelles, such as chloroplasts and mitochondria, they can still perform the functions of these organelles by localizing certain metabolic enzymes on:
a. the nuclear membranes.
b. the endoplasmic reticulum.
c. infoldings of the plasma membrane.
d. ribosomes.
e. the cell wall
C. In folding of the plasma membranes
Extrachromosomal circles of DNA in bacteria called ______ often carry genes involved in _______:
a. capsids, bacterial reproduction
b. capsids, antibiotic resistance.
c. plasmids, viral resistance.
d. plasmids, antibiotic resistance
D. Plasmids, antibiotic resistance
Many Gram (+) Eubacteria like Clostridium botulinum and Bacillus anthraxis have the ability to survive harsh environmental conditions by protecting their DNA in a dormant structure called a(n)
a. capsule
b. endospore
c. endotoxin
d. sporangium
e. capsid
B. Endospore
Once they appeared on Earth, ___ forever changed the atmosphere on earth by the production of largequantities of oxygen gas.
a. Gm (+) eubacteria
b. archaebacteria
c. cyanobacteria
d. plants
e. Gm (-) rickettsias
C. Cyanobacteria
Prokaryotes found inhabiting the Great Salt Lake would be the _____.
a. cyanobacteria
b. extreme halophiles
c. methanogens
d. extreme thermophiles
B. Extreme halophiles
The structure in the accompanying figure labeled C (outermost layer) is___, and the structure indicated by the letter I is _____:
a. C - the plasma membrane, I - the cilia.
b. C - the capsule, I - the cilia
c. C - the capsule, I - the flagella
d. C - the cell wall, I - the flagella
e. C - the capsule, I - the pilus
C. C- the capsule, I- the flagella
What is the function of the structures labeled B?
a. to adhere to host cells, or other bacteria, or solid substrates
b. to prevent phagocytosis by a white blood cell of the host
c. to transmit DNA from one cell to another
d. to exchange genetic material and genetic traits like antibiotic resistance between cells
e. All answers except B
All answers except B
The ____ Archaebacteria are typically found in extremely hot environments, whereas the _______Archaebacteria are typically found in swampy environments lacking oxygen.
a. Extreme halophile, methanogenic
b. Extreme thermophile, methanogenic
c. Pyrrhanogen, Extreme halophilic
d. Methanogens, Extreme thermophilic
e. Gram positive, Gram negative.
B. Extreme Thermophile, methanogenic
Organisms that share the most DNA homology to mitochondria and chloroplasts, respectively, are:
a. Proteobacteria, cyanoacteria.
b. Proteobacteria, green sulfur bacteria.
c. Eubacteria, green sulfur bacteria.
d. Eubacteria, cyanobacteria.
e. E. coli, green sulfur bacteria
A. Proteobacteria, Cyanobacteria
What makes rRNA (or another sequence) a good 'evolutionary chronometer'?a. It is universally distributed across the group chosen - all organisms have rRNA.
b. It is functionally similar between organisms - rRNAs all participate in protein synthesis.
c. Its sequence changes slowly - good for looking across long periods of time
d. The rRNA sequences can be aligned, or matched up, between 2 organisms.
e. All of the above
All of the above
The most significant difference between the Archaea and the Eubacteria is:
a. the lack of a nuclear envelope in the Archaea.
b. the absence of the 70S ribosomes in the Eubacteria.
c. the presence of a single filament flagellum in the Eubacteria.
d. the small subunit (16 S) rRNA sequence.
e. All of the above
D. The small subunit (16 S) rRNA sequence
The first indication that Archaea were different from the Eubacteria came from:
a. the absence of peptidoglycans in the cell walls of the Archaea.
b. Ribosome size.
c. 16 S rRNA sequence.
d. nuclei.
e. response to antibiotics
A, the absence of peptidoglycans in the cell walls of Archaea
Most bacterial cells keep from bursting in a hypotonic environment by
a. an efficient water pump
b. a tough cell membrane.
c. pumping large quantities of salts into the cell.
d. a rigid cell wall.
e. a stiff capsule
D. A rigid cell wall
Gram-positive bacteria stain _____ in a gram stain because of a thick layer of _____ in their cell walls
a. green; peptidoglycan
b. purple; peptidoglycan
c. green; cellulose
d. purple; polysaccharides
e. red; polysaccharides
B. Purple; peptidoglycan
Which of the following groups of prokaryotes contains the genus Clostridium, responsible for the human diseases tetanus and botulism?
a. Spirochetes
b. Cyanobacteria
c. Methanogens
d. Gram Positive Eubacteria
e. Proteobacteria
D. Gram Positive eubacteria
Which of the following groups of prokaryotes in the figure above contains the bacteria Helicobacter pylori, responsible for human ulcers, and E. coli, beneficial intestinal bacteria in humans?
a. Thermophiles
b. Cyanobacteria
c. Methanogens
d. Gram Positive Eubacteria
e. Proteobacteria
E. Proteobacteria
Which of the following groups of prokaryotes might be found in the Great Salt Lake, the Red Sea, the DeadSea, but NOT necessarily in the ocean?
a. Cyanobacteria
b. Methanogens
c. Gram Positive Eubacteria
d. Proteobacteria.
e. Extreme Halophiles
E. Extreme halophiles
SELECT ALL THAT APPLY Which of the following cellular structures are shared by bacteria, archaea, andeukaryotes?
a. Plasma membrane
b. Nuclear envelope
c. Membrane-bound organelles
d. Cell wall composed of peptidoglycan
e. Linear chromosomes
A plasma membrane
Match the Prokaryotes to their classification group:
Prokaryotes that thrive in the Great Salt Lake and salt evaporation ponds
1. Cyanobacteria
2. Halophiles
3. Gram negative proteobacteria
4. Thermophiles
2. Halophiles
Match the Prokaryotes to their classification group:
Prokaryotes that thrive in extremely hot environments
1. Cyanobacteria
2. Halophiles
3. Gram negative proteobacteria
4. Thermophiles
Thermophiles
Match the Prokaryotes to their classification group:
Prokaryotes most closely related to human mitochondria
1. Cyanobacteria
2. Halophiles
3. Gram-negative proteobacteria
4. Thermophiles
Gram negative Proteobacteria
Match the Prokaryotes to their classification group:
Prokaryotes most closely related to sunflower chloroplasts
1. Cyanobacteria
2. Halophiles
3. Gram negative proteobacteria
4. Thermophiles
Cyanobacteria
Clostridium tetani
Tetanus (“lockjaw”)
Clostridium botulinum
Botulism - food poisoning