Unit 4: Biodiversity Flashcards

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1
Q

What is biodiversity?

A

Biodiversity is the variety of living organisms in an area. Includes:
- Species diversity: the number of different species and the abundance of each species in an area. (E.g. wood contains many different species like plants, insects, birds, and mammals)
- Genetic diversity: the variation of alleles within a species (or a population of a species like human blood types determined by a gene with three different alleles)
A population is a group of organisms of the same species living in a particular area.

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2
Q

What is endemism?

A

It is when species is unique to a single place and it isn’t usually found anywhere else in the world. E.g. the giant tortoise is endemic to the Galapagos Islands and can only be found there.

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3
Q

What is natural selection?

A

Adaptation and evolution has increased biodiversity, but human activities are reducing species diversity- causing biodiversity to fall.

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4
Q

What is conservation?

A

Help maintain biodiversity. Important for endemic species because they are particularly vulnerable to extinction as they can’t migrate number will decline.

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5
Q

How can species diversity be measured?

A

Count the number of different species within the area- species richness. Higher the number of species, the greater the species richness but gives no indication of the abundance of each species.
Count the number of different species and the individuals within a species (they have different alleles) - index of diversity.
To do this use a random sample

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6
Q

What is genetic diversity?

A

It is the variety of alleles in the gene pool of a species. Gene pool is the complete set of alleles in a species. Greater the variety of alleles, the greater the genetic diversity. You can look over time or look at two populations of the same species show different diversity.

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7
Q

What is the phenotype?

A

Observable characteristic. Different alleles code for slightly different versions of the same characteristics. Looking at different phenotypes in a population of a species, you can get an idea of the diversity of alleles. Large number of different phenotypes= greater genetic diversity

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8
Q

What is the genotype?

A

Sequence of the base pairs analysed, Order of bases in DNA is slightly different. You can look at similarities and differences. Measure the number of different alleles to see how genetically diverse. The larger the number of different alleles, the greater the genetic index.
Heterozygosity index:
H= number of heterozygotes/ number of individuals in the population

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9
Q

How to calculate the index of diversity?

A

It takes into account both the number of species and the abundance.
D=N(N-1)/ Sum of n(n-1)
N= total number of organisms of all species
n= total number of organisms of one species
Higher the number the more diverse it is. Compare species diversity in different habitats.

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10
Q

What is the niche?

A

Role organism plays in the community. Interactions between living and non-living organisms. Unique niche- can only be occupied by one species. There can be slight differences. If two species try to occupy the same niche, they will compete, the more successful one will be left.

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11
Q

How can an animal adapt?

A

Behavioural- ‘play dead’ or dance before mating like scorpions.
Physiological- Brown bears hibernate
Anatomical (structural)- Otter have a streamlined shape.

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12
Q

How do adaptations become more common with evolution?

A

Mutations introduce new alleles- variation in their phenotypes. Determine characteristics that can make an individual more likely to survive.
Selection pressures such as predation, disease, competition- struggle for survival. Individual without advantageous alleles don’t survive- fewer individuals- less competition for resources. Individuals who are adapted more likely to survive- reproduce and pass on to offspring. Over time the number of individuals with advantageous alleles increase. Evolution- favourable adaptations become more common. Charles Darwin. Example: Peppered moths

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13
Q

What is speciation?

A

Species is a group is similar organisms that can reproduce to giver fertile offspring. Speciation is the development of a new species. Occurs when the same species become reproductively isolated (due to geographical isolation or a random mutation) - changes in the alleles and phenotypes of the population prevent them from successfully breeding together. Changes include:
- Seasonal change- mating season
- Mechanical changes- changes in the genitalia
- Behavioural changes- courtship rituals

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14
Q

How can geographical isolation lead to speciation?

A

Physical barrier divides population- volcanic eruption, floods, earthquakes. Conditions will be different- different climates. This causes different characteristics to become more common due to natural selection- different selection pressures.
Characteristics will be advantageous on each side, allele frequency will change, increase on one side. Mutations will take place independently, changing allele frequency. lead to changes in phenotypes frequencies. They will become genetically distinct- won’t be able to produce fertile young- reproductively isolated

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15
Q

Why is the Hardy-Weinberg equation used?

A

Used to estimate the frequency of particular alleles, genotypes and phenotypes within a population.

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16
Q

What is the Hardy-Weinberg equation to predict the allele frequency?

A

p + q = 1
p= frequency of dominant allele
q= frequency of recessive allele

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17
Q

What is the Hardy-Weinberg equation to predict the phenotype and genotype frequency?

A

p^2 + 2pq + q^2 = 1
p^2= frequency of homozygous dominant genotype
2pq= frequency of heterozygous genotype
q^2= frequency of homozygous recessive genotype

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18
Q

What does classification do?

A

Taxonomy is the science behind classification. Organising them into groups between their similarities and differences. It goes Domain, Kingdom, Phylum, Class, Order, Family, Genus, Species. Different species can’t produce fertile young. Same species- very similar. Based on their phenotype, genotype and how related they are.

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19
Q

What are the 5 kingdoms?

A

Prokaryotae (bacteria), Protoctista (algae), Fungi (moulds), Plantae (mosses), Animalia (fish)

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20
Q

What can new data do to classification?

A

Influence the way it is classified. It can lead to an organism being reclassified or lead to changes in the classification system structure. Tentative nature

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21
Q

How to zoos and seedbanks help to conserve endanger species?

A

Extinction of a species or loss of genetic diversity causes a reduction in global biodiversity. Endangered species - risk of extinction because of low population or a threatened habitat. Conservation involves the protection and management of endangered species. Zoos and seed banks help to conserve endangered species and genetic diversity.

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22
Q

What is a seedbank?

A

Stores seeds from lots of different species of plants- endangered. Used to grow new plants. Conserve genetic diversity. Range of seeds from plants with different characteristics- different alleles.
Involves:
- Cool, dry conditions- long time
- Testing seeds for viability (ability to grow plant)- planted, grown, new seeds are harvested
Pros:
- Cheaper, Large number, Less labour, stored anywhere, less likely to be damaged by disease
Cons:
- Testing for viability is expensive and time- consuming and for all plants to be tested, difficulty to collect seeds from remote locations

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23
Q

What is a zoo?

A

Captive breeding programmes. Controlled environment. Endangered species or extinct in the wild- bred together - increase numbers. However, problems breeding outside their natural habitats, hard to recreate, people may think it is cruel.

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24
Q

What can zoos and seed banks do?

A

Reintroduce the species and increase their numbers back in the wild and bring them back from the brink of extinction. Help organisms that rely of these plants and animals or as part of their habitat. Restoring habitats that have been lost. Problems:
- Bring new disease, harming organisms that already live there
- May not behave as they would if they were raised in the wild

25
Q

How do seedbanks and zoos contribute to scientific research?

A

Seedbanks: how plant species can be successfully grown from seed and reintroduce them to the wild. Medical research, new crops, new materials, don’t have to remove endangered species. Disadvantage: small, interbred population, may not be representative.
Zoos: increase knowledge of behaviour, physiology, nutrition needs can contribute. Carry out research that is not possible in the wild. Disadvantage: animals in captivity may act differently

26
Q

How do seedbanks and zoos educate people?

A

Zoos can get people enthusiastic about conservation work.
Seedbanks can provide training and set up local seed banks

27
Q

What is a cell wall?

A

Surrounds plant cells. Made mainly of carbohydrate cellulose. Supports the plant

28
Q

What is the middle lamella?

A

Outermost layer of the cell. Adhesive- sticking adjacent plant cells together- gives the plant stability

29
Q

What is plasmodesmata?

A

Channels in the cell walls that linked adjacent cells together. Transports substances and communicates between cells

30
Q

What are pits?

A

Region in the cell wall where the wall is very thin. Arranged in pairs. Lined up to puts on adjacent cells. Transports substances between cells

31
Q

What are chloroplasts?

A

Small, flattened structure, surrounded by a double membrane called thylakoid membranes. These membrane are stacked up in some parts of the chloroplasts to form grana- linked together by the lamellae- thin, flat pieces of thylakoid. It is the site of photosynthesis

32
Q

What are amyloplasts?

A

Small organelle enclosed by a membrane. They contain starch granules. Storage of starch grains- convert starch back to glucose for release when the plants need it.

33
Q

What are the vacuole and tonoplasts?

A

Vacuole is a compartment surrounded by a membrane called the tonoplast. Vacuole contains the cell sap (made up of water, enzymes, minerals and waste). Vacuole keeps the cell turgid- stops it from wilting. Involved in the breakdown and isolation of unwanted chemicals in the cell. Tonoplasts controls what enters and leaves the vacuole.

34
Q

What is the xylem vessel?

A

Transports water, mineral ions and provides support. Long, tube-like formed by dead cells, bundles. Long, wide, hollow lumen (no cytoplasm), no end walls, uninterrupted tube. Walls are thickened with the woody substance lignin- supports the plant. Water and mineral ions move in and out of the vessel through pits in the walls where there is no lignin.

35
Q

What is the sclerenchyma fibres?

A

Provide support, made of bundles of dead cells, long, wide, hollow lumen but have end walls. Walls thickened with lignin but don’t have pits. They have more cellulose than other plant cells

36
Q

What are phloem fibres?

A

Transports organic solutes from where they are made to where they are needed (translocation). Cells arranged in tubes- transport tissue. Contains sieve tube elements (allows solutes to pass through- no nucleus, very thin layer of cytoplasm, few organelles- can’t survive without companion cells) and companion cells (carries out living functions for themselves and their sieve cells- provide energy for active transport)

37
Q

Where are the xylem vessels, phloem tissue, and sclerenchyma fibres in the stem?

A

Xylem vessels group together with phloem tissue to form vascular bundles. Sclerenchyma fibres are usually associated with the vascular bundles

38
Q

How to dissect a plant stem?

A

1)Cut a thin section of the plant stem using a scalpel. Take care when using the sample and remember to cut away from you.
2)Place the tissue sample into water to prevent it from drying out.
3)Place the tissue sample into a small dish containing the stain. A common stain that is used to view vascular bundles is toluidine blue O (TBO) which stains lignin blue/green which will enable you to visualise the xylem and sclerenchyma fibres. The phloem cells and remaining tissue will appear a pink/purple colour.
4)Rinse the tissue samples in water and place each one onto a microscope slide.

39
Q

What is cellulose?

A

Long, unbranched chain of beta glucose joined by 1-4 glycosidic bonds, straight, between 50 to 80 cellulose chains are linked together by a large number of hydrocarbon bonds to form strong threads called microfibrils- provides structural support

40
Q

Why are plant fibres strong?

A

The cell wall contains cellulose microfibrils in a net-like arrangement. The strength of the microfibrils and their arrangement in the cell wall gives plant fibres strength. Some structural plant cells produce a secondary cell wall- thicker usually contains more lignin. Growth of a secondary cell wall in called secondary thickening- make plant fibres even stronger

41
Q

How do you measure the tensile strength?

A
  1. Use the scalpel to remove 9 fibrous strings from your plant sample, examining each one to
    check there are no breakages along its length and that its diameter is even.
  2. On the chopping board or white tile use a ruler and scalpel to cut the 9 strings into three 10
    cm, three 15 cm and three 20 cm lengths.
  3. Set up the clamps and retorts as shown in the diagram with the first 10 cm string.
  4. Ensure the string is properly secured with the cotton wool cushioning directly beneath it,
    then begin to add weights to the string, 10g at a time until the string breaks. Record the
    mass added in your results table.
  5. Repeat step 4 with each of the other two 10 cm strings and calculate a mean mass added
    before the string breaks. Then repeat for the 15 cm and 20 cm lengths.
  6. Plot a graph of your results.
42
Q

What are sustainable practices?

A

Using resources that need to be present for suture generations - sustainability. Renewable resources to make products sustainable. Used indefinitely without running out- regrown unlike fossil fuels - unsustainable practice can’t continue indefinitely and will run out

43
Q

How can plant fibres be used to make sustainable products?

A

Ropes and fabrics made out of plastic but can be made out of plant fibres. More sustainable- less fossil fuel- regrown and maintain supply for future generations. Biodegradable by microbes. Easier to grow and process- makes them cheaper.

43
Q

How can plant fibres be used to make sustainable products?

A

Ropes and fabrics made out of plastic but can be made out of plant fibres. More sustainable- less fossil fuel- regrown and maintain supply for future generations. Biodegradable by microbes. Easier to grow and process- makes them cheaper.

44
Q

How can starch be used to make sustainable products?

A

Plastics are made from oil, but some can be made from plant-based materials like starch called bioplastics - more sustainable - crops - regrown. Vehicle fuel usually made from oil but can be made from starch - bioethanol. More sustainable- less fossil fuels- crops and regrown

45
Q

Why is water needed?

A

Photosynthesis, transport material, structural rigidity, regulate temperature

46
Q

Why is magnesium ions needed?

A

production of chlorophyll- pigment needed for photosynthesis

47
Q

Why are nitrate ions needed?

A

Production of DNA, proteins and chlorophyll, plant growth, fruit production, seed production

48
Q

Why are calcium ions needed?

A

Important components in plant cell wall. Required for plant growth

49
Q

Experiment to investigate plant mineral deficiencies in the lab

A
  1. Use the measuring cylinder to fill test tubes with each of the nutrient solutions.
  2. Cover the top of the test tube with tinfoil. Poke a hole through the tinfoil.
  3. Push the roots of the Bryophyllum plantlets through the hole in the tinfoil into
    the solution.
  4. Wrap the test tubes in tinfoil (to prevent light getting in) and place them under a
    sunny window.
50
Q

Experiment to investigate plants antimicrobial properties

A
  1. Carry out aseptic techniques detailed above.
  2. Crush 3g of the garlic and mint (separately) with methylated spirit. Shake
    occasionally.
  3. Use a sterile pipette to transfer plant extract to paper disc.
  4. Leave paper discs to dry for 10 minutes.
  5. Use sterile forceps to place the paper disc onto a petri dish.
  6. Lightly tape a lid on, invert and incubate at 25°C for 24 hours. DO NOT tape
    around the entire dish as this prevents oxygen entering and so promotes the
    growth of more harmful anaerobic bacteria.
  7. Sterilise equipment used to handle bacteria and disinfect work surfaces.
  8. Measure the diameter of the inhibition zone (clear circle) for each plant. DO
    NOT remove lid from agar plate.
  9. Work out the area of the inhibition zone using the formula:
    A= (pie) d / 4
    where d is the diameter.
    NB: Bacteria sample is incubated at 25°C as incubating at 37°C (human body
    temperature) could enable pathogens to grow that are harmful to humans.
51
Q

How to make sure the conditions are right for bacterial growth?

A

Source of nutrients, (if rely on aerobic respiration)- supply of oxygen, temperature and pH- affect enzyme activity

52
Q

What are some aseptic techniques?

A

Prevent contamination of cultures by unwanted microorganisms- human pathogens.
- Close windows and doors
- disinfect
- sterile safety equipment and discard safely and pre-sterile plastic instrument are used once
- Work near a Bunsen flame as hot air rises
- Briefly flame the neck of the glass container of broth- opened and closed. Prevents unwanted organisms from falling in

53
Q

How was drug testing in the past?

A

Using trial and error and it was a lot less scientific

54
Q

What is drug testing like today?

A

Phase 1- small group, healthy individuals, safe dosage- any side effects, how the body reacts
Phase 2- drug passes phase 1, use a larger group and see how well the drug actually works
Phase 3- compare drug to existing treatments, use hundreds or even thousands of patients- more reliable. Patients split into 2 groups- 1 gets the older treatment and the other get the new one

55
Q

What is the placebo?

A

Inactive substance- doesn’t do anything. Placebo effect- show some improvement because they believe they are getting treated. Allows researchers to see how the drug actually works.

56
Q

What is the placebo?

A

Inactive substance- doesn’t do anything. Placebo effect- show some improvement because they believe they are getting treated. Allows researchers to see how the drug actually works.

57
Q

What is the double blind study design?

A

Neither the doctor or the patient knows who has the new drug or the placebo- to avoid bias in the results because the attitudes of the patient and the doctors can’t effect the results