UNIT 2 (topic 1) microscopes Flashcards

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1
Q

negatives of light microscope

A

low resolution

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2
Q

why does the light microscope have low resolution

A
  • wavelength of light is too long
  • light diffracts when it passes through the gap of a similar length to its wavelength
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3
Q

definition of magnification

A

how many times larger an image appears

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4
Q

definition of resolution

A

minimum distance between two objects before they appear as one

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5
Q

negatives of the electron microscope

A
  • image in black and white
  • specimens must be dead
  • electrons from objects illuminate a screen, where it is visualised - expensive
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6
Q

negatives and positives of TEM

A
  • very high resolution
  • 3D image formed
  • thin specimen required
  • must be dead
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