Unit 2: Microscopy

Question 1

Two types of microscope

Answer 1

Electron
Light

Question 2

Two types of electron microscope

Answer 2

Scanning (SEM)
Transmission (TEM)

Question 3

Advantages/Disadvantages of TEM

Answer 3

Advantages - Provide high resolution images

Disadvantages- Specimen need to be sliced thinly.

Question 4

How does staining enable cell components to become visible?

Answer 4

Stains increase the contrast as different components within the cells takes up the stain to varying degrees, enabling components to become visible.

Question 5

What are the advantages and disadvantages of light microscopes?

Answer 5

Increase in magnification and resolution, both living and non-living, less magnification than electron

Question 6

What are the advantages and disadvantages of electron microscopes?

Answer 6

Advantages: higher magnification and resolution than light, subcellular level
Disadvantages: non-living only, larger, more expensive, maintenance