UNIT 2: EQUIPMENT Flashcards
- Used when there is a need to
culture microorganisms and
for substances that must be
protected from contamination - Used to make broth, agar
deep, and agar slant
SCREW CAPPED TUBES
- Used to detect production of
gas by microorganisms. An
inverted small tube is initially
filled with the solution in
which the microorganism is
to be grown
DURHAM FERMENTATION TUBE
- Simpler than Durham tube.
Used also for observing
microorganisms perform
fermentation.
SMITH FERMENTATION TUBE
- used to make agar
plates for microbiology
studies
PETRI DISH
- Glassware calibrated for
precise volume. - Used for measuring or
storing different
amounts of liquid
VOLUMETRIC FLASK
- Has a single graduation
that allows it to deliver
one specific volume
accurately
VOLUMETRIC PIPETTE
- serological or blow-out
pipettes have no 0ml
mark as that corresponds
to an empty pipette
SEROLOGIC PIPETTE
- Makes it easy to deliver
liquids, especially small
volumes, accurately and
precisely - Has a disposable tip
PIPETTOR
- Used to observe
specimens for a long time
because they do not dry
up very quickly. Thus,
motility of an organism
can be observed.
HANGING DROP SLIDE
- AKA smear loop, inoculation
wand or microstreaker - used to retrieve and transfer
an inoculum from a culture of
microorganisms
INOCULATING LOOP
- mainly used to pick a single
colony (pure) and transfer in an
agar slant (stab and streak
method)
INOCULATING NEEDLE
- Test tube rack
- Slant rack
- Staining rack
RACKS
- Uses dry heat to sterilize
heat-stable materials - For sterilization of media
and other materials
affected by moisture
HOT AIR STERILIZER
- Uses moist heat or steam
under pressure - For sterilization of media
and other materials
affected by moisture
AUTOCLAVE
- Not sterile and cannot
be used to sterilize
culture media - For drying specimens
and lab apparatus or
glassware
DRYING OVEN
- used for growing
bacterial cultures or
providing suitable
conditions for a
chemical or biological
reaction
INCUBATOR
- It is used in the
microbiological lab for
incubations or enabling
chemical reactions to
occur at higher
temperatures
WATER BATH
- Storage for culture media
- Preserves the
morphological
characteristics of
microorganisms while
arresting their growth
.REFRIGERATOR
- An enclosed, ventilated
lab workspace for safely
working with pathogens
requiring a defined
biosafety level
BIOSAFETY CABINET (BSC)
Microbes not known to consistently cause disease in immunocompetent adult humans, of the minimal potential hazard to laboratory personnel and the environment
Example; skin bacteria, yeast
BSL-1
Microbes pose moderate potential hazard to personnel and environment
Example: Herpes simplex, common cold viruses (RSV, rhinovirus), salmonella
BSL-2
Indigenous or exotic microbes that may cause serious or potentially lethal disease via inhalation.
Example: Tuberculosis, SARS-CoV 2, highly pathogenic avian influenza, plague (yersinia pestis)
BSL-3
Exotic agents that pose a high risk of aerosol-transmitted laboratory infections and life-threatening disease that is frequently fatal, for which there are no vaccines or treatments.
Example: Ebola virus, small pox
BSL-4
