Unit 1- Lecture 3

Question 1

Why is the double helix perfect for dna replication?

Answer 1

-The strands can separate and the original strand will also be encoded which serves as the template for DNA replication

Question 2

Semi conservative

Answer 2

Has 1 old strand of DNA and 1 new strand of DNA

Question 3

Conservative

Answer 3

Has 1 double helix that is old and 1 double helix that is new

Question 4

Meselson-Stahl Experiment

Answer 4

-Labeled old DNA strand with N15 and new DNA strand with N14
-Ultra centrifuged them–>N15 sunk to bottom, N14 floated to top

Question 5

Can we replicate DNA in a test tube? And what do we need in test tube

Answer 5

YES
1. Single stranded DNA
2. DNA polymerase
3. dNTP
4.Primers

Question 6

Where does DNA replication get the energy that is required for the process?

Answer 6

From the triphosphate group on the nucleotides

Question 7

What direction is DNA polymerization

Answer 7

-5’ to 3’
-adds to OH group of 3’ end of growing strand