Unit 1 - KA2 - DNA Replication Flashcards
Direction of new nucelotides being added to parent strand.
5’-3’ direction
Strand of DNA which is copied to produce a new strand.
Template
Small sequence of single stranded DNA required to start (prime) DNA replication.
Primer
Copying a molecule of DNA
Replication
Method used by a cell to replicate DNA to produce two daughter DNA molecules, each containing one original and one new strand of DNA.
Semi conservative
Continuous replication of one strand of DNA from one primer.
Leading Strand
Discontinuous replication of DNA as the double strand of DNA is unzipped to reveal more of the DNA read in the 3’ to 5’ direction.
Lagging Strand
Enzyme required to join newly replicated fragments of DNA formed by discontinuous DNA replication together.
Ligase
Point at which the DNA strands are unzipped for DNA replication to take place.
Replication fork
End of parent strand that DNA replicates (attaches to)
3’ (it moves 5’ - 3’)
Enzyme which adds complementary nucleotides to the deoxyribose 3’ end of a DNA strand
DNA Polymerase
Repeated heating and cooling of a DNA template.
Thermal Cycling - PCR
Producing a very large number of copies of a DNA fragment from possibly only one copy of DNA.
Amplification
Method of amplifying DNA sequences by repeatedly copying the region between two primers.
Polymerase Chain Reaction (PCR)
A type of DNA polymerase that is still active at relatively high temperatures.
Taq Polymerase
