Types of Microscope

Question 1

What was first used to view microscopic objects?

Answer 1

In 1500’s, magnifying glasses were used.

Question 2

Describe the first microscope

Answer 2

Two convex lenses at either end of the barrel.

Question 3

What discovery made using a microscope led to the term cell?

Answer 3

Robert Hooke, 1660’s used an early compound microscope to view the structure of cork, leading to the term cell (cells in a prison).

Question 4

Describe light microscopes

Answer 4

Light source passes through specimen, through lenses to reach viewer. Magnification up to 1500x. Resolution up to 0.2 micro metres. Specimen can be living or dead.

Question 5

Describe fluorescence microscopes

Answer 5

Sample labelled with fluorescent substance that attaches to the specific structures in the cell unable to be seen by light microscope. Sample illuminated with high-intensity light

Question 6

Describe electron microscopes

Answer 6

Uses an electron beam, and electromagnets rather than lenses. Produces a computer image. Have a high resolving power (0.0002 micro metres). Magnification up to 1,500,000x. Specimen must be dead.
Two types:
Scanning EM - electrons reflected off specimen, 3D image
Transmission EM - electrons pass through, 2D

Question 7

Describe confocal laser imaging

Answer 7

Confocal laser scanning microscopes use pinpoint laser beams to make images of many layers, which are combined into a 3D model.