Types Of Chromotography Flashcards
1
Q
Thin layer chromatography
A
Nonpolar solvent
Polar card
Identify sample
2
Q
Reverse phase
A
Polar solvent
Nonpolar card
Identify sample
3
Q
Column chromatography
A
Nonpolar solvent
Polar gel or solvent
Separate sample into components
4
Q
Ion exchange
A
Nonpolar solvent
Charged beads
Separate components by charge
5
Q
Size exclusion
A
Nonpolar solvent
Polar porous beads
Separate components by size
6
Q
Affinity chromatography
A
Nonpolar solvent
Beads coated with antibody or receptor for target molecule
Purify molecule
7
Q
Gas chromatography
A
Inert gas
Crushed metal or polymer
Separate vaporizable compounds
8
Q
HPLC
A
Nonpolar solvent
Small column with concentration gradient
Similar to column but more precise