Types Of Chromotography

Question 1

Thin layer chromatography

Answer 1

Nonpolar solvent
Polar card
Identify sample

Question 2

Reverse phase

Answer 2

Polar solvent
Nonpolar card
Identify sample

Question 3

Column chromatography

Answer 3

Nonpolar solvent
Polar gel or solvent
Separate sample into components

Question 4

Ion exchange

Answer 4

Nonpolar solvent
Charged beads
Separate components by charge

Question 5

Size exclusion

Answer 5

Nonpolar solvent
Polar porous beads
Separate components by size

Question 6

Affinity chromatography

Answer 6

Nonpolar solvent
Beads coated with antibody or receptor for target molecule
Purify molecule

Question 7

Gas chromatography

Answer 7

Inert gas
Crushed metal or polymer
Separate vaporizable compounds

Question 8

HPLC

Answer 8

Nonpolar solvent
Small column with concentration gradient
Similar to column but more precise