Types Of Chromotography Flashcards

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1
Q

Thin layer chromatography

A

Nonpolar solvent
Polar card
Identify sample

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2
Q

Reverse phase

A

Polar solvent
Nonpolar card
Identify sample

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3
Q

Column chromatography

A

Nonpolar solvent
Polar gel or solvent
Separate sample into components

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4
Q

Ion exchange

A

Nonpolar solvent
Charged beads
Separate components by charge

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5
Q

Size exclusion

A

Nonpolar solvent
Polar porous beads
Separate components by size

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6
Q

Affinity chromatography

A

Nonpolar solvent
Beads coated with antibody or receptor for target molecule
Purify molecule

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7
Q

Gas chromatography

A

Inert gas
Crushed metal or polymer
Separate vaporizable compounds

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8
Q

HPLC

A

Nonpolar solvent
Small column with concentration gradient
Similar to column but more precise

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