Types of Antibodies used in IHC Flashcards
Types of antibodies used in IHC: (2)
- Polyclonal
- Monoclonal
Antibodies produced by different cells
Polyclonal
Monoclonal antibodies reacts with various epitopes. Animal source of monoclonal antibodies are mice.
a. First statement is true, second statement is true.
b. First statement is true, second statement is false.
c. First statement is false, second statement is true.
d. First statement is false, second statement is false.
c. First statement is false, second statement is true.
Polyclonal antibodies react with various epitopes. Monoclonal antibodies only with one specific type of epitope.
This is part of the antigen that reacts with the antibody
Epitopes
Main source of Polyclonal antibodies for IHC
Rabbits
Other sources of polyclonal antibodies: (3)
Goat, Pigs, Sheep
This antibody is more specific and produced from individual clone of plasma cell.
Monoclonal
Source of monoclonal antibodies:
Mice