Specimen of IHC

Question 1

This section is requested for rapid diagnosis, when px is in the OR.

Answer 1

Frozen section

Question 2

How is frozen section carried out?

Answer 2

Intra-operatively

Question 3

Preparation for frozen section:

Answer 3

Cut the tissue specimen using either the freezing microtome or cryostat (more commonly used today)

Question 4

Microtome used in frozen sections: (2)

Answer 4

Freezing microtome
Cryostat.

Question 5

Fixative used when tissues are prepared as cryostat section: (2)

Answer 5

Methanol or Acetone

Question 6

Purpose of fixation of frozen section: (2)

Answer 6

1. To preserve the position of antigens being
   detected
2. To prevent destruction of labile antigenic sites
   (which are binding sites for Ab)

Question 7

Preferred fixative for IHC

Answer 7

Formaldehyde

Question 8

Which specimens are required to use antigen retrieval?

Answer 8

1. Formalin fixed spx
2. Paraffin embedded spx