Detection Using Labeled Enzyme Antibody

Question 1

What is the reaction of detecting labelled enzyme antibody?

Answer 1

Ag + Ab → End Product Detection (LM)

Question 2

Involves the use of only one antibody that is labeled. The labeled antibody is added to the antigen being detected. End product is observed under the Light Microscope.

Answer 2

Direct IHC

Question 3

A more sensitive method. Modification of the direct/ traditional method. This involves addition of dextran polymer

Answer 3

Enhanced Polymer One-Step Staining Method (EPOS)

Question 4

More sensitive than the direct method. A two-step method. Requires the use of 2 antibodies

Answer 4

Indirect IHC

Question 5

Antibodies required in IHC: (2)

Answer 5

1. Primary antibody
2. Secondary antibody

Question 6

This antibody used in Indirect IHC will attach to the Fc portion of the primary antibody, thereby increasing sensitivity

Answer 6

Secondary antibody

Question 7

This antibody used in Indirect IHC is specific to antigen being detected

Answer 7

Primary antibody