Tripple Sugar Iron (TSI) agar slant slant

Question 1

TSI agar test

Answer 1

Differentiates among the different the Enterobacteriaceae from other gram- intestinal bacilli

Question 2

Enterobacteiacaea

Answer 2

TSI slant
all gram– bacilli
glucose fermenters with production of acid

Question 3

Alkaline slant acid butt

Answer 3

TSI slant
with/out gas production (breaks in agar butt)
red slant
yellow butt
only glucose fermentation occurred

Question 4

Acid slant acid butt

Answer 4

TSI salnt
with/out gas production
yellow slant 
yellow butt
Lactose and/or sucrose fermentation occurred.

Question 5

Alkaline slant alkaline butt

Answer 5

TSI slant
red slant
red or red orange (No change) butt
No carbohydrate fermentation occurred.

Question 6

H2S

Answer 6

TSI slant

will show an extensive blackening in the butt because of the precipitation of the insoluble sulfide.