Transport in plants Flashcards

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1
Q

Why do plants need a transport system

A

-every cell of multicellular plants need a regular supply of oxygen, water, nutrients and minerals
-plants are not very active and respiration rate is low therefore demand for oxygen is low - can be met by diffusion
-demand for water and sugar is high - plants can absorb water and minerals at roots but sugar can’t be absorbed from soil
-leaves can perform gaseous exchange and manufacture sugars by photosynthesis but can’t absorb H20 from air therefore water and minerals from roots up to leaves and sugars from leaves to rest of plant

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2
Q

What are the different types of vascular tissue

A

-xylem tissue in which water and soluble mineral ions travel upwards
-phloem tissue in which assimilates such as sugars travel up or down
-there is no pump and respiratory gases are not carried by these tissues

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3
Q

Describe how xylem and phloem are distributed in young root

A

-vascular bundle found at centre of young root
-central core of xylem, often in shape of X
-phloem found in between arms of x-shaped xylem tissue
-this arrangement provides strength to withstand pulling forces to which roots are exposed
-around vascular bundle is special sheath of cells called endodermis - key role in getting water into xylem vessels
-just inside endodermis is layer of meristem cells, that can divide, called pericycle

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4
Q

Describe distribution of xylem and phloem in the stem

A

-vascular bundles are found near the outer edge of the stem
-non-woody plants have bundles separate and discrete
-woody plants the bundles are separate in young stems but become a continuous ring in older stems - means there is a complete ring of vascular tissue just under bark of tree; this arrangement provides strength and flexibility to withstand bending forces to which stems and branches are exposed
-xylem is found towards inside each vascular bundle and phloem towards outside
-in between phloem and xylem is layer of cambium - layer of meristem cells that divide to produce new xylem and phloem

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5
Q

Describe distribution of xylem and phloem in the leaf

A

-vascular bundle form the midrib and veins of a leaf
-a dicotyledonous leaf has a branching network of veins that gets smaller as spread away from midrib
-within each vein, xylem located on top of phloem

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6
Q

Structure and function of xylem

A

-transports water and mineral ions from roots up to leaves
-vessels to carry water and dissolved mineral ions
-fibres to help support plant
-living parenchyma cells which act as packing tissue to separate and support vessels

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7
Q

Describe the xylem vessels

A

-as xylem vessels develop, lignin impregnates walls of cells, killing them and making them waterproof
-end walls and contents decay leaving column of dead cells with no contents - tube called xylem vessels
-lignin strengthens the vessel walls and prevents collapse - keeps them open even during short water supply
-the patterns of lignin prevent vessel from being too rigid and allows some flexibility of stem or branch
-when lignification not complete, gaps left in cell wall - bordered pits form
-bordered pits in 2 adjacent vessels are aligned to allow water to leave one vessel and pass to another - also allow water to leave xylem and pass into living parts

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8
Q

How are xylem vessels adapted to carry water and mineral ions from roots to top of plant

A

-made from dead cells, end to end, forming continuous column
-narrow tubes, water column doesn’t break easily and capillary action effective
-bordered pits in lignified walls allow water move sideways from one vessel to another
-lignin deposited in walls in spiral, annular or reticulate patterns allows xylem to stretch as plant grows and enables stem/branch to bend

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9
Q

How is flow of water not impeded (prevented) in xylem

A

-no cross walls
-no cell contents, nucleus or cytoplasm
-lignin thickening prevents walls from collapsing

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10
Q

Structure and function of phloem

A

-phloem is a tissue used to transport assimilated around the plant
-sucrose is dissolved in water to form sap
-phloem tissue consists of sieve tubes, made up of sieve tube elements, and companion cells

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11
Q

Describe sieve tube elements

A

-elongated sieve tube elements are lined up, end to end, to form sieve tubes - contain no nucleus and very little cytoplasm, which leaves space for mass flow of sap to occur
-ends of sieve tube elements are perforated cross walls - sieve plates, which allow movement of sap from one element to another
-sieve plates have pores which very rapidly become blocked by deposition of callose to prevent loss of sap and inhibits transport of pathogens after injury or infection

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12
Q

Describe companion cells

A

-between sieve tubes are small cells, each with a large nucleus and dense cytoplasm - these are companion cells
-have numerous mitochondria to produce ATP needed for active processes - companion cells carry out metabolic processes needed to load assimilated actively into sieve tubes
-companion cells and sieve tube elements in phloem linked by strands of cytoplasm - allows communication and flow substances between the cells

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13
Q

Compare xylem and phloem

A

XYLEM
-impregnaned with lignin - supports, prevents collapse, waterproofs the walls
-end walls decay so water can flow unimpeded
-small diameter, narrow, allows for capillary action
-leaves long column of continuous, hollow, dead cells - minimises obstruction to water
PHLOEM
-made of sieve tube elements, have very little cytoplasm and no nucleus
-have companion cells which have large nucleus and dense cytoplasm and lots of mitochondria to produce ATP to load sucrose
-sieve plates with pores to allow flow of sap
-plasmodesmata links cytoplasm of companion cells to phloem

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14
Q

Define hydrophyte

A

-plant adapted to living water or where the ground is very wet

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15
Q

Define xerophyte

A

-plant adapted to living in dry conditions

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16
Q

How are terrestrial plants (land) adapted to reduce water loss (behavioural and structural)

A

-waxy cuticle on leaf - reduce water loss due to evaporation through epidermis
-stomata often found on under surface of leaves not top - reduces evaporation due to less evaporation by direct heating from sun
-most stomata close at night when no light for photosynthesis
-deciduous plants lose leaves in winter when ground may be frozen (and water less available) and when temperatures too low for photosynthesis

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17
Q

Why is marram grass adapted

A

-ammophila specialises in living on sand dunes
-conditions are particularly harsh because water in sand drains away quickly, sand may be salty and leaves are exposed to windy conditions
-marram grass is a xerophyte

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18
Q

How is marram grass adapted

A

-leaf rolled longitudinally so air is trapped inside - this air becomes humid. reducing water loss from leaf
-thick waxy cuticle on outer side of rolled leaf to reduce evaporation
-stomata on inner side of rolled lead so are protected by enclosed air space
-stomata are in pits in lower epidermis, also folded and covered by hairs - reduce air movement and therefore loss of water vapour
-spongy mesophyll is very dense with few air spaces so less surface area for evaporation

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19
Q

How are cacti adapted

A

-cacti are succulents - they store water in their stems which become fleshy and swollen
-stem often ribbed or fluted so it can expand when water available
-leaves are reduced to spines - reduces surface area of leaves therefore less water lost be transpiration
-stem green to allow photosynthesis
-roots very widespread in order to take advantage of any rain that does fall

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20
Q

Describe other features of xerophytes

A

-closing stomata when water availability low which reduces water loss and reduces need to take up water
-some plants have low water potential inside their leaf cells which is achieved by maintaining high salt concentration in the cells
-low water potential reduces evaporation of water from cell surface as water potential gradient reduced
-very long tap root that can reach water deep underground

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21
Q

Why and how are hydrophytes adapted

A

-hydrophytes have easy access to water however they struggle to get oxygen to their submerged tissues and keep afloat
-they have many large air spaces in leaf to keep them afloat - therefore in air and can absorb sunlight for photosynthesis
-stomata on upper epidermis so they’re exposed to air allowing for gaseous exchange
-leaf stem has many large air spaces - helps with buoyancy but allows oxygen to diffuse quickly to roots for aerobic respiration

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22
Q

Describe water uptake and movement across the root

A

-outermost layers of cells (epidermis) of a root contains root hair cells - cells with a long extension, increasing surface area
-these cells absorb mineral ions and water from soil
-water moves across root cortex down a water potential gradient to endodermis of vascular bundle
-water may also travel through apoplast pathway as far as endodermis but then must enter symplast pathway as apoplast blocked by casparian strip

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23
Q

Summarise how it root absorption works

A

-mineral ions actively absorbed from soil making water potential of cytoplasm more negative
-water enters root hair cell by osmosis
-water move across root cortex by osmosis via apoplast pathway
-mineral ions actively transported into medulla making water potential more negative so water follows by osmosis

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24
Q

Describe role of endodermis

A

-movement of water across root is driven by active process that occurs at endodermis
-endodermis is layer of cells surrounding medulla and xylem - also known as starch sheath as contains granules of starch - a sign energy is being used

25
Q

Role of casparian strip

A

-casparian strip blocks apoplast pathway between cortex and medulla
-made of suberin
-ensures that water and dissolved mineral ions have to pass into cytoplasm through plasma membrane
-plasma membrane contain transporter proteins, actively pump minerals ions from cytoplasm of cortex into medulla and xylem - makes water potential of medulla and xylem more negative so water moves in by osmosis
-once water enters medulla, cannot pass back into cortex due to apoplast blocked by casparian strip

26
Q

How is water moved up the stem

A

-movement of water up the xylem is by mass flow
-this is known as the flow of water and mineral ions in the same direction
-it requires root pressure, transpiration pull and capillary action

27
Q

Describe root pressure

A

-pressure in root medulla builds up and forces water into xylem, pushing water up the xylem
-root pressure can push water a few metres up the stem but cannot account for water getting to top of tall trees

28
Q

Describe transpiration pull

A

-loss of water by evaporation from leaves must be replaced by water coming up from xylem
-cohesion forces are strong enough to hold the molecules together in a long chain or column
-as molecules are lost at top of column, the whole column is pulled up as one chain
-pull from above creates tension in column of waters - lignin prevents the vessel from collapsing under tension
-mechanism involves cohesion between water molecules and tension in column of water; called cohesion tension theory
-relies on plant maintaining unbroken column of water all way up xylem -if one vessel broken, the water column can still be maintained through another vessel via bordered pits

29
Q

Describe capillary action

A

-Some forces that hold water molecules together also attract the water molecules to the sides of the xylem vessel - adhesion
-xylem vessels are very narrow, these forces of attraction can pull water up sides of the vessel

30
Q

How does water leave the leaf

A

-water leaves the leaf as water vapour through stomata
-only tiny amount leaves through the waxy cuticle
-water evaporates from cells living the cavity immediately above guard cells (sub stomatal air space)
-lowers the water potential in these cells, causing water to enter them by osmosis from neighbouring cells
-in turn water drawn from xylem in leaf by osmosis - may also reach cells by apoplast pathway from xylem

31
Q

Describe the plasmodesmata

A

-cellulose cell walls of a plant cell are fully permeable to water
-water molecules can also pass across cell wall and through partially permeable membrane to cytoplasm/vacuole
-many plant cells joined by special cytoplasmic bridges - these are cell junctions at which cytoplasm of one cell connected to that of another through gap in cell walls - called plasmodesmata

32
Q

Describe apoplast pathway

A

-water passes through spaces in cell walls and between cells
-does not pass through any plasma membrane
-means water moves by mass flow rather than by osmosis - dissolved mineral ions and salts can be carried with the water

33
Q

Describe symplast pathway

A

-water enters cell cytoplasm through plasma membrane
-it can pass through plasmodesmata from one cell to next
-osmosis is occurring

34
Q

Describe vacuolar pathway

A

-similar to symplast pathway but water is not confined to cytoplasm of cells
-it is able to enter and pass through vacuoles as well

35
Q

How does water move from cell to cell in terms of water potential

A

-water always moves from a region of high water potential to low
-in a plant cell, cytoplasm contains mineral ions and sugars that will reduce water potential - because fewer free water molecules available than in pure water
-as a result, water potential in plant cells is always negative

36
Q

Describe water uptake in plant cells

A

-if place a plant cell in pure water it will take up water molecules by osmosis because water potential in cell is more negative than water potential of water
-water molecules move down water potential gradient into cell but cell will not absorb water till it bursts because cell has a strong cellulose cell wall
-once cell is full of water it is described as turgid
-water inside cell starts to exert pressure on cell wall and is called pressure potential
-as pressure potential builds up it reduces influx of water

37
Q

Describe water loss in plant cells

A

-if plant cell placed in salt solution with very negative water potential it will lose water by osmosis - because water potential of cell is less negative than water potential of solution therefore water moves down water potential gradient and out of cell
-as water loss continues the cytoplasm and vacuole shrink
-cytoplasm no longer pushes against cell wall and cell no longer turgid - if water continues to leave cell, plasma membrane will lose contact with wall -plasmolysis
-tissue is now flaccid

38
Q

Describe transpiration

A

-transpiration is the loss of water vapour from upper parts of the plant - particularly the leaves
-some water may evaporate through upper leaf surface but this loss is limited by waxy cuticle
-most water vapour leaves through stomata which open to allow gaseous exchange for photosynthesis
-photosynthesis occurs when there is sufficient light therefore majority water vapour lost during the day

39
Q

Describe the typical pathway taken by most water molecules leaving the leaf

A
  1. water enters leaf through xylem and moves by osmosis into cells of spongy mesophyll. It may also pass along cell walls via apoplast pathway
  2. Water evaporates from cell walls of spongy mesophyll
  3. Water vapour moves by diffusion out of leaf through open stomata. This relies on difference in concentration of water vapour molecules in leaf compared to outside of leaf - known as water vapour potential gradient. Must be higher water vapour potential inside lead than outside as diffuses down the gradient.
40
Q

What is the importance of transpiration

A

-transpiration is essential for plant to survive as water vapour lost from leaf must be replaced from below - draws water up stem as a transpiration stream
-this transports useful mineral ions up the plant
-supplies water for growth, cell elongation and photosynthesis
-maintains cell turgidity
-supplies water that as it evaporates can keep the plant cool on a hot day

41
Q

FACTORS AFFECT TRANSPIRATION : light intensity

A

-in light, stomata open to allow gaseous exchange for photosynthesis
-higher light intensity increases transpiration rate

42
Q

FACTORS AFFECT TRANSPIRATION: temperature

A

-higher temperatures= increased rate of transpiration
-increase rate of evaporation from cell surfaces so that water vapour potential in leaf rises
-increase rate of diffusion through stomata because water molecules have more kinetic energy
-decrease relative water vapour potential in air allowing more rapid diffusion of molecules out of leaf

43
Q

FACTORS AFFECT TRANSPIRATION: relative humidity

A

-higher relative humidity in air will decrease rate of water loss
-because smaller water vapour potential gradient between air spaces in lead and air outside

44
Q

FACTORS AFFECT TRANSPIRATION: air movement/wind

A

-air moving outside lead will carry away water vapour that has just diffused out of leaf
-this maintains high water vapour potential gradient

45
Q

FACTOES AFFECT TRANSPIRATION: water availability

A

-if little water in soil, plant cannot replace water that is lost
-if sufficient water in soil, stomata close and leaves wilt

46
Q

How is transpiration rate calculated

A

volume/time

47
Q

What are other factors affecting the rate of transpiration

A

-number of leaves - more leaves will increase the rate of transpiration
-number, size and position of stomata
-presence of waxy cuticle

48
Q

How do hydrophytes transpire

A

-if water cannot leave the plant the transpiration stream stops and plant cannot transport mineral ions up to leaves
-many plants contain specialised structures at tips or margins of leaves called hydathodes - these structures can release water droplets which may then evaporate from leaf surface

49
Q

Describe what translocation is

A

-translocation occurs in the phloem and is the movement of assimilates throughout the plant
-assimilates are substances made by plant using substances absorbed from the environment
-this includes sugars, sucrose and amino acids
-part of plant that loads assimilates into phloem sieve tubes called source
-part of plant that removes assimilates from phloem sieve tubes is called a sink

50
Q

Describe active loading

A

-Sucrose is loaded into sieve tube by active process - involves use of energy from ATP in companion cells
-energy used to actively transport hydrogen ions out of the companion cells - increases their concentration outside cells and decreases concentration inside companion cells therefore a concentration gradient is created
-hydrogen ions diffuse back into companion cells through special cotransporter proteins - these proteins allow movement of hydrogen ions into cell if accompanied by sucrose molecules - known as cotransport
-also called secondary active transport as results from active transport of hydrogen ions out of cell and moves sucrose against concentration gradient
-as concentration of sucrose in companion cell increases it can diffuse through plasmodesmata into sieve tube

51
Q

Describe the movement of sucrose

A

-movement of sucrose along phloem is by mass flow
-solution of sucrose, amino acids and other assimilates flow along the tube -called sap, can flow up or down the plant
-flow is caused by difference in hydrostatic pressure between two ends of tube; produces pressure gradient
-water enters tube at source increasing pressure and leaves tube at sink reducing pressure - sap flows source to sink

52
Q

Describe the source

A

-sucrose entering sieve tube elements makes water inside sieve tube lower - therefore water molecules move into sieve tube element from surrounding tissues by osmosis
-this increases hydrostatic pressure in sieve tube at source
-source is any part of plant that loads sucrose into sieve tube - most obvious is leaf
-sugars made during photosynthesis are converted to sucrose and loaded into phloem sieve tubes

53
Q

Describe the sink

A

-a sink is anywhere that removes sucrose from the phloem sieve tubes
-the sucrose could be used for respiration and growth in a meristem or it could be converted to starch for storage in a root
-where sucrose is being used in the cells, it can diffuse out of sieve tube via plasmodesmata - may also be removed by active transport
-removal of sucrose from sap makes water potential less negative so water moves out of sieve tube into surrounding cells- this reduces hydrostatic pressure in phloem at sink

54
Q

Describe what happens along the phloem

A

-water entering sieve tube at source increases hydrostatic pressure
-water leaving sieve tube at sink reduces hydrostatic pressure
-therefore a pressure gradient is set up along the sieve tube and sap flows from higher pressure to lower pressure
-this could be in either direction depending upon where sap is being produced and where it is needed
-it is even possible that sap could be flowing in opposite directions in different sieve tubes at same time
-since sap in one tube is all moving in same direction - this is mass flow

55
Q

Steps for translocation at source

A
  1. Hydrogen ions actively transported for companion cell to source using ATP
  2. In source cell, glucose made from photosynthesis combined with fructose to make sucrose
  3. cotransporter proteins allows hydrogen ions to diffuse back into companion cell. Sucrose diffuse through cotransporter protein at same time
  4. sucrose diffuses from companion cell to sieve tube element via plasmodesmata
  5. water potential in sieve tube element decreases
  6. water moves down a water potential gradient from xylem to sieve tube elements by osmosis
  7. causes increase in hydrostatic pressure in sieve tube element
    8.this produces hydrostatic pressure gradient
    9.phloem liquid containing assimilates moves by mass flow down hydrostatic pressure gradient
56
Q

Steps for translocation at sink

A
  1. sucrose is used or converted to starch
  2. concentration gradient from sieve tube element to sink cell for sucrose
  3. sucrose diffuses from sieve tube element to sink cell via plasmodesmata
  4. increase in water potential in sieve tube element
  5. water moves out of sieve tube element by osmosis down water potential gradient
  6. lowers hydrostatic pressure in sieve tube element
  7. this is what produces hydrostatic pressure gradient
57
Q

Where is there evidence for mass flow

A

-an aphid will stick its mouth part into plant to access sugar
-the phloem contains sucrose and this is evident as this is where aphid sticks mouth into

58
Q

How do we know energy is used

A

-additional mitochondria in companion cells to produce ATP
-translocation is stopped if inhibitors used to stop formation of ATP