Exchange surfaces and Transport in animals Flashcards

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1
Q

NEED FOR EXCHANGE SYSTEM: size

A

-in very small organisms, such as singled celled organisms, cytoplasm very close to environment in which they live
-diffusion supply enough oxygen and nutrients to keep cells alive
-multicellular organisms may have several layers of cells
-any oxygen or nutrients diffusing in from outside have longer diffusion pathway therefore insufficient

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2
Q

NEED FOR EXCHANGE SYSTEM: surface area to volume ratio

A

-small organisms have small surface area and small volume -meaning their surface area to volume ratio large and all cells can be supplied with sufficient oxygen
-larger organisms have larger surface area but also larger volume - they have smaller surface area to volume ratio therefore need an exchange system

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3
Q

NEED FOR EXCHANGE SYSTEM: level of activity

A

-some organisms are more active than others
-metabolic activity uses energy from food and requires energy released from oxygen in aerobic respiration
-cells of an active organism need good supplies of nutrients and oxygen to supply energy for movement
-this needs for energy is increased in those animals such as mammals, that keep themselves warm

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4
Q

Features of a good exchange system

A

-large surface area to provide more space for molecules to pass though
-thin barrier to reduce diffusion pathway - must be permeable to substances being exchanged
-good blood supply to maintain steep concentration gradient so diffusion can occur rapidly

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5
Q

How is gas exchanged in the lungs

A

-gases pass by diffusion through thin walls of alveoli
-oxygen passes from air in alveoli to blood in capillaries
-carbon dioxide passes from blood to air in alveoli
-lungs must maintain steep concentration gradient to ensure diffusion continues
-alveoli provide a very large surface area and are lined by thin layer of moisture which evaporates and is lost as we breathe out
-alveoli also produce surfactant that coats internal surface of alveoli to reduce cohesive forces between water molecules as these forces tend to make alveoli collapse

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6
Q

Describe permeability of exchange barrier

A

-barrier to exchange is comprised of wall of alveolus and wall of blood capillary
-cells and plasma membranes readily allow diffusion of oxygen and carbon dioxide as molecules small and non-polar

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7
Q

Why is exchange barrier thin

A

-alveolus wall is one cell thick
-capillary wall one cell thick
-both walls consist of squamous cells - flattened and very thin
-capillaries in close contact with alveolus walls
-capillaries so narrow that red blood cells squeezed against capillary wall - reducing rate of flow

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8
Q

Why does exchange have good blood supply

A

-helps maintain steep concentration gradient
-blood system transport carbon dioxide from tissues to lungs; ensures concentration of CO2 in blood is higher than in air - CO2 diffuses into alveoli
-blood transports oxygen away from lungs - ensures that concentration of O2 in blood kept lower than that in alveoli so oxygen diffuses into blood

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9
Q

What is ventilation

A

-ventilation ensures that concentration of oxygen in air of alveolus remains higher than that in blood; concentration of carbon dioxide in alveoli remains lower than that in blood - concentration gradient necessary so diffusion maintained

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10
Q

Steps of inspiration

A

1) diaphragm contracts to move don and become flatter displacing digestive organs down
2) external intercostal muscles contract to raise ribs
3)volume chest cavity increased
4)pressure chest cavity drops below atmospheric pressure
5)air moved into lungs

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11
Q

Steps of expiration

A

1) diaphragm relaxes and pushed up
2) external intercostal muscles relax and ribs fall
3)volume chest cavity decreased
4) pressure in lungs increases and rises above pressure in atmosphere
5) air is moved out of the lungs

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12
Q

Describe lung tissue

A

-lungs consist of large numbers of tiny air-filled sacs called alveoli - compromised of squamous epithelium and are surrounded by blood capillaries so diffusion pathway short
-alveolus walls contain elastic fibres that stretch during inspiration but then recoil to help push air out during expiration

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13
Q

What are the requirements of the airways

A

-trachea, bronchi and bronchioles allow passage of air into lungs in and out again
-be large enough to allow sufficient air to flow without obstruction
-be supported, preventing collapse when air pressure is low during inspiration
-be flexible in order to allow movement

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14
Q

How are airways lined

A

-airways lined by ciliated epithelium - goblet cells in epithelium release mucus, trapping pathogens
-cilia then move mucus up to top of airway where swallowed
-glandular tissue in loose tissue also produces mucus

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15
Q

Describe structure of trachea and bronchi

A

-these airways supported by rings of cartilage which prevent collapse during inspiration
-rings of cartilage in trachea are C shaped allowing flexibility and space for food to pass down oesophagus

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16
Q

Describe structure of bronchioles

A

-larger bronchioles have some cartilage but smaller ones have none
-wall is compromised mostly of smooth muscle and elastic fibres
-smallest bronchioles end in clusters of alveoli

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17
Q

Describe the tissues in bronchus

A

-goblet cell secretes mucus which increases dust and bacteria
-ciliated epithelium; cilia move mucus along airway
-smooth muscle contracts to constrict airway
-loose tissue containing elastic fibres, glands and blood vessels
-small blood vessels
-cartilage supports airway preventing collapse

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18
Q

Describe smooth and elastic tissue

A

-smooth muscle contract, constricting airway
-makes lumen of airway narrower, restricting flow of air to and from alveoli - controlling this is important if there are harmful substances in air
-contraction of smooth muscle and control of airflow is not voluntary and may be result of allergic reaction
-once smooth muscle contracted, cannot reverse on its own
-when is contracts it deforms elastic fibres
-as muscle relaxes elastic fibres recoil to original size and shape, dilating airway

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19
Q

How is lung volume measured

A

-lung volumes can be measured using spirometer
-a float-chamber spirometer consists of a chamber of air or medical grade oxygen floating on a tank of water
-during inspiration air is drawn from chamber so lid moves down
-during expiration air returns to chamber, raising lid
-movements may be recorded on data logger
-carbon dioxide rich air exhaled is passed through chamber of soda-lime, absorbing carbon dioxide - this allows measurement of oxygen consumption

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20
Q

Precautions when using a spirometer

A

-subject should be healthy, free from asthma
-soda-lime should be fresh and functioning
-should be no air leaks in apparatus as this gives inaccurate results
-mouthpiece should be sterilised
-water chamber must not be overfilled
-use nose clip

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21
Q

Describe the lung volumes

A

-vital capacity is maximum volume of air that can be moved by lungs in one breath
-vital capacity is affected by size of person, age and gender and their level of regular exercise
-vital capacity typically 2.5-5dm3 but may rise in trained athletes
-residual volume is volume of air that remains in lungs after forced expiration - approximately 1.5dm3
-tidal volume is volume of air moved in and out with each breath
-usually measured at rest - typically 0.5dm3

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22
Q

How is oxygen uptake measured

A

-breathing supplies oxygen for respiration and removes carbon dioxide
-as person breaths from spirometer, oxygen absorbed by blood and replaced by carbon dioxide
-carbon dioxide absorbed by soda lime in spirometer so that volume of air in chamber decreased
-this decrease can be observed and measured on spirometer trace
-we can assume volume of carbon dioxide released and absorbed by soda lime equals volume of carbon dioxide absorbed by blood
-therefore measuring gradient of decrease in volume enables us to calculate rate of oxygen uptake

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23
Q

How is breathing rate measured

A

-breathing rate can also be measured from spirometer trace - simply count number of peaks in a minute
-breathing rate at rest usually 12-14 breaths per minute
-oxygen uptake will depend upon number of factors
-increase oxygen uptake will result from increased breathing rate of deeper breaths

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24
Q

Describe the exchange system of bony fish

A

-bony fish must exchange gases with water in which they live
-they use gills in order to absorb oxygen dissolved in water and release carbon dioxide in eater
-oxygen concentration typically much lower than air
-most bony fish have 5 pairs gills which are covered by bony plater called operculum
-each gill consist of two rows of gill filaments attached bony arch
-filament are very thin and surface folded into secondary lamellae providing large surface area
-blood capillaries carry deoxygenated blood close to surface of secondary lamellae where exchange takes place

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25
Q

Describe the counter-current flow

A

-if did not have counter current flow, blood would reach equilibrium
-blood flows along gill arch and out along filaments to secondary lamellae
-blood flows through capillaries in opposite direction to the flow of water over lamellae
-this arrangement creates counter current flow that absorbs maximum amount of oxygen from water via steep concentration gradient

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26
Q

Describe the ventilation in bony fish

A

-bony fish can keep water flowing over gills by using buccal opercular pump
-the buccal cavity can change volume
-floor of mouth moves downwards drawing water into buccal cavity
-mouth closes and flow raised again pushing water through gills
-movements of operculum are coordinated with movements of buccal cavity - as water pushed through buccal cavity, operculum moves outwards
-this movement reduces pressure in opercular cavity, helping water to flow through gills

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27
Q

Describe the exchange system of insects

A

-insects do not transport oxygen in blood
-haemolyph not in vessels
-insects have open circulatory system in which the body fluid acts as both blood and tissue fluid
-circulation slow and affected by body movements
-insects possess an air filled tracheal system which supplies air directly to all respiring tissues
-air enters system via pore in each segment called spiracle
-air is transported into body through series of tubes called tracheae - these divide into smaller tubes called tracheoles
-ends of tracheoles are open and filled with fluid called tracheal fluid
-gaseous exchange occurs between air in tracheole and tracheal fluid - some exchange can occur across thin walls of tracheoles
-many insects very active and need good supply oxygen
-when tissues active, tracheal fluid can be withdrawn into body fluid to increase surface area- more oxygen absorbed when insect active

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28
Q

Describe the ventilation in insects

A

-in many insects, sections of tracheal system are expanded and have flexible walls - these act as air sacs which can be squeezed by action of flight muscles
-repetitive expansion and contraction of these sacs ventilated the tracheal system
-in some insects, movement of wings alter volume of thorax as thorax volume decreases, air in tracheal system is put under pressure and pushed out; when thorax volume increases, pressure inside drops and air pushed into tracheal system
-some insects have developed this ventilation even further

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29
Q

How is ventilation of locusts specialised

A

-locusts can alter volume of abdomen by specialised breathing
-these are coordinated with opening and closing valves in spiracles
-as abdomen expands, spiracles at front end of body open and air enters tracheal system
-as abdomen reduces in volume, spiracles at rear end of body open and air can leave tracheal system

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30
Q

Why do animals need transport system

A

-larger animals with complex anatomy will have more than two layers of cells therefore diffusion distance becomes too long and diffusion alone will be too slow to meet requirements:
-supply of oxygen and nutrients for growth and survival
-removal of waste products so they don’t build up to become toxic

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31
Q

Why does size affect transport system

A

-cells inside large organism are further from its surface - diffusion pathway increased
-diffusion rate is reduced and diffusion too slow to supply all requirements
-also outer layer of cells use up supplies, so less will reach cells deep inside body

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32
Q

How does surface area to volume ration affect transport system

A

-larger animals have smaller surface area to volume ration
-each gram of tissue has smaller area of body surface for exchange

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33
Q

How does level of metabolic activity affect need for transport system

A

-animals need energy from food so they can move around
-releasing energy from food by aerobic respiration requires O2
-if animal very active, its cells need good supplies of nutrients and oxygen to supply energy for movement
-animals that keep themselves warm, need even more energy

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34
Q

Features of an effective transport system

A

-a fluid or medium to carry nutrients, oxygen and waste around body - the blood
-a pump to create pressure that will push the fluid around body - this is heart
-exchange surfaces that enable substances to enter blood and leave it again where needed - this is capillaries

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35
Q

Features of an efficient transport system

A

-tubes or vessels to carry blood by mass flow
-two circuits - one to pick up oxygen and another to delivery oxygen to tissues

36
Q

Compare fish and animal circulatory system

A

-fish have a single circulatory system -the blood flows through heart once for each circuit of body
-mammals have double circulatory system - system has two separate circuits

37
Q

Describe the double circulatory system of mammals

A

-one circuit carries blood to the lungs to pick up oxygen - pulmonary circuit
-the other circuit carries the oxygen and nutrients around the body to the tissues - systemic circulation
-blood flows around heart twice; heart - body - heat - lungs - heart

38
Q

Compare the single and double circulatory system

A

DOUBLE
-blood pressure is higher
-rate of blood flow is higher
-delivery of oxygen and glucose is higher

39
Q

What is the advantage of a double circulatory system in mammals

A

-mammals are active and maintain body temperature
-supplying energy for activity and heat needed to keep body warm requires energy from food - released in process of respiration
-to release a lot of energy, cells need a good supply of both nutrients and oxygen as well as removal of waste products

40
Q

What are open circulatory systems

A

-means that blood is not always held within blood vessels
-blood circulates through body cavity so tissues and cells are bathed in blood
-in some animals, movements of body help circulate the blood - without movement, transport of oxygen and nutrients stops
-in some animals, there is muscular pumping organ
-blood from body enters heart through pores called ostia
-heart pumps blood toward head by peristalsis
-at forward end of heart, blood pours into body cavity, circulation can continue when insect at rest but body movements affect circulation
-some larger and more active insects have open ended tubes attached to heart - these direct blood towards active parts of body

41
Q

Disadvantages of open circulatory systems

A

-blood pressure is low and blood flow is slow
-circulation of blood may be affected by lack of movement

42
Q

Describe a closed circulatory system

A

-delivers oxygen directly to all cells
-in larger animals, blood stays entirely inside vessels
-separate fluid called tissue fluid bathes tissues and cells

43
Q

Advantages of closed circulatory system

A

-higher pressure so blood flows more quickly
-more rapid delivery of oxygen and nutrients
-more rapid removal of carbon dioxide and other waste products
-transport is independent of body movements

44
Q

Structure of arteries

A

-arteries carry blood away from heart
-blood is at high pressure so artery wall must be thick in order to withstand pressure
-lumen relatively small to sustain high pressure
-inner wall folded to allow lumen to expand as blood flow increases
-endothelium reduces friction
-walls consist of three layers
-inner layer consists of thin layer of elastic tissue which allows wall to stretch and recoil to maintain blood pressure
-middle consists thick layer of smooth muscle which constricts to increase blood flow
-outer layer relatively thick layer of elastic tissue providing strength for high pressure

45
Q

Structure of arterioles

A

-small blood vessels that distribute blood from artery to capillary
-arteriole walls contain layer of smooth muscle
-contraction of muscle will constrict diameters of arteriole
-this increases resistance to flow and reduces rate of blood flow
-constriction of arteriole walls can be used to divert flow of blood in regions of body that demand more oxygen

46
Q

Structure of capillaries

A

-have very thin walls - allow exchange of materials between blood and tissue fluid
-lumen very narrow, 7um- red blood cells may be squeezed against walls of capillary; helps transfer oxygen as reduces diffusion pathway to tissues
-increases resistance and reduces rate of flow
-walls consist of single layer of flattened endothelial cells - reduces diffusion distance
-walls are leaky - allow blood plasma and dissolved substances to leave blood through fenestrations

47
Q

Structure of venules

A

-from capillaries, blood flows into small vessels called venules
-collect blood from capillary bed and lead into veins
-consist of thin layers of muscle and elastic tissue
-outside endothelium and thin outer layer of collagen

48
Q

Structure of veins

A

-lumen relatively large to ease flow of blood
-walls have thinner layers of collagen, smooth muscle and elastic tissue than in artery walls
-don’t need to stretch and recoil and not always constricted in order to reduce blood flow
-contain valves to help blood flow back to heart and prevent flowing in opposite direction
-walls are thin so vein can be flattened by action of surrounding skeletal muscles
-contraction surrounding skeletal muscle applies pressure to blood, forcing it to move in direction determined by valves

49
Q

What is blood plasma

A

-blood is the fluid held in our blood vessels
-it consists a liquid called plasma containing many blood cells
-plasma contains dissolved substances including oxygen, carbon dioxide, minerals, glucose, hormones and amino acids
-cells include RBC, various WBC and platelets

50
Q

What is tissue fluid

A

-tissue fluid is similar to blood plasma but doesn’t contain most cells found in blood neither does it contain plasma proteins
-tissue fluid formed by plasma leaking from capillaries - it surrounds cells in tissues and supplies them with oxygen and nutrients
-as blood plasma leaks from capillary, carries dissolved substances into tissue fluid - movement by mass flow
-waste products from cell metabolism will be carried back into capillaries

51
Q

Describe the formation of tissue fluid

A

-when artery reaches tissues, it branches into smaller arterioles then into network of capillaries
-eventually link up with venules to carry blood back to veins, therefore blood flowing into organ or tissue is contained in capillaries
-at aerial end of capillary, blood at relatively high hydrostatic pressure - this pressure tend to push fluid out of capillaries through capillary wall
-fluid can leave through tiny gaps between cells in capillary wall
-the fluid that leaves blood consists of plasma with dissolved nutrients and oxygen
-all red blood cells, platelets and most WBC remain in blood as well as plasma proteins
-tissue fluid surrounds body cells so exchange of gases and nutrients can occur across plasma membranes
-exchange occurs by diffusion, facilitated diffusion and active uptake

52
Q

How does tissue fluid return to the blood

A

-blood pressure at venous end of capillary is much lower
-this allows some tissue fluid to return to capillary carrying carbon dioxide and other waste products into blood

53
Q

What happens to tissue fluid that does not re-enter blood

A

-some tissue fluid directed into another tubular system called lymph system
-drains excess tissue fluid out of tissues and returns it to blood system in subclavian vein in chest
-fluid in lymphatic system called lymph and has similar composition to tissue fluid
-contains more lymphocytes as these are produced in lymph nodes
-lymph nodes are swellings found at intervals along lymph system - play important role in immune system

54
Q

Compare the features of blood plasma, tissue fluid and lymph

A

BLOOD PLASMA
-high hydrostatic pressure
-more negative oncotic pressure
-RBC, neutrophils, lymphocytes
-plasma proteins
-transported in lipoproteins
TISSUE FLUID
-low hydrostatic pressure
-less negative oncotic pressure
-some neutrophils especially in infected areas
-few proteins
-few fats
LYMPH
-low hydrostatic pressure
-less negative oncotic pressure
-lymphocytes
-few proteins
-more fats, especially near digestive system

55
Q

Describe the movement of fluids

A

-tissue has its own hydrostatic pressure and oncotic pressure of solutes also has influence on movement of fluids
-hydrostatic pressure of blood tends to push fluid out of tissues
-hydrostatic pressure of tissue tends to push fluid into capillaries
-oncotic pressure of blood tends to pull water back into blood
-oncotic pressure of tissue fluid pulls water into tissue fluid

56
Q

Describe the external features of the heart

A

-in humans heart is just off centre towards left of chest cavity
-main part consists of cardiac muscles
-two main pumping chambers - ventricles and atria
-lying over surface of heart there are coronary arteries which supply oxygenated blood to heart muscle
-arteries very important to heart as its hard working - if they become constricted, can have severe consequences to health of heart organ
-restricted blood flow reduces delivery of oxygen and nutrients - may cause myocardial infarction or angina
-at top of heart there are veins that carry blood into atria and arteries that carry blood away from heart

57
Q

Describe the internal features of the mammalian heart

A

-heart divided into 4 chambers
-upper chambers are atria - these receive blood from major veins
-deoxygenated blood from body flows through vena cava into right atrium
-oxygenated blood from lungs flows through pulmonary vein to left atrium
-from atria blood flows down atrio-ventricular valves to ventricles
-action attached to valves are tendinous cords which prevent calves from turning inside out when ventricle walls contract
-wall of muscle called septum separates ventricles from each other
-this ensures oxygenated blood in left side of heart and deoxygenated blood in right side kept separate

58
Q

Describe the flow of heart

A

-deoxygenated blood leaving right ventricle flows into pulmonary artery leading to lungs where its oxygenated
-oxygenated blood leaving left ventricle flows into aorta
-carries blood to number of arteries that supply all part of body
-at base of major arteries where they exit heart are semilunar valves - prevent blood returning to valves as ventricles relax

59
Q

Blood pressure of aorta

A

-muscle of atrial walls very thin
-this is because chambers do not need to create much pressure
-their function is to receive blood from veins and push it to ventricles

60
Q

Bkood pressure of right ventricle

A

-walls of right ventricle thicker than walls of atria
-enables right ventricle to pump blood out of heart
-right ventricle pumps deoxygenated blood to lungs
-lungs in chest cavity beneath heart so blood does not need to travel very far
-also alveoli in lungs very delicate and could be damaged by very high blood pressure

61
Q

Blood pressure of left ventricle

A

-walls of left ventricle two or three times thicker than those of right
-blood from left ventricle pumped out through aorta and needs sufficient pressure to overcome resistance of systemic circulation

62
Q

Describe the structure of cardiac muscle

A

-cardiac muscle consists of fibres that branch producing cross bridges
-these help spread stimulus around heart and ensure muscle can produce squeezing action rather than simple reduction in length
-numerous mitochondria between muscle fibrils to supply energy for contraction
-muscle cells separated by intercalated discs that facilitate synchronised contraction
-each cell has nucleus and its divided into contractile units called sarcomeres
-has visible stripes/striated
-myogenic - can initiate its own contraction and doesn’t need any communication with brain

63
Q

What is the cardiac cycle

A

-role of heart is to create pressure that pushes blood around blood vessels
-muscular walls of 4 chambers must contract in coordinating sequence which allows heart to fill with blood before pumping it away

64
Q

Describe the cardiac cycle

A

VENTRICULAR SYSTOLE
-both right and left ventricles pump together
-contraction starts at apex of heart so blood is pushed up towards arteries
DIASTOLE
-muscular walls of all 4 chambers relax
-elastic recoil causes chamber to increase in volume allowing blood to flow in from veins
ATRIAL SYSTOLE
-both right and left atria contract together
-muscle in walls is thin so only small increase in pressure is created
-this helps push blood into ventricles stretching their walls and ensuring they are full of blood

65
Q

Describe the pressure in the blood vessels

A

-the artery walls close to heart have lot of elastic tissue
-when blood leaves the heart these walls stretch
-as blood moves out of aorta, pressure in aorta starts to drop
-elastic recoil of walls helps maintain blood pressure in aorta
-further the blood flows along the arteries, the more pressure drops and fluctuations become less obvious
-it is important to maintain pressure gradient between aorta and arterioles as this is what keeps blood flowing towards the tissues

66
Q

Interpret the pressure over time diagram of the heart

A

1) pressure in left ventricle greater than pressure in left atrium
-beginning of ventricular systole
-AV valves close
2) pressure in left ventricle greater than pressure in aorta so SL valves open
3)ventricle wall starts to relax
-pressure in ventricle smaller than pressure in aorta
-SL valves close
4) pressure in ventricles smaller than pressure in left atrium so AV valves open

67
Q

What is normal blood pressure

A

-systolic = ventricles contracting
-120mmHg
-diastolic -atria and ventricles relaxed
-80mmHg

68
Q

How do you calculate heart rate

A

-heart beat length / 60

69
Q

Describe diastole

A

-atria and ventricles relax
-blood flows into atria from veins
-pressure in atria greater than pressure in ventricles therefore AV valves open
-pressure in arteries greater than pressure in ventricles so SL valves close

70
Q

Describe atrial systole

A

-walls of left and right atria contract
-empties blood from atria into ventricles

71
Q

Describe ventricular systole

A

-all valves closed momentarily
-as walls of ventricles contract
-pressure in ventricles greater than pressure arteries so open SL valves

72
Q

How is the cardiac cycle coordinated

A

-the sinoatrial node in the wall of the right atrium
-the SAN is the hearts pacemaker, dictates heart rate
-is small patch of tissue that sends out waves of electrical excitation at regular intervals to initiate synchronised contraction
-wave of excitation passed through AVN, down septum in bundle of HIS
-arrives at apex, speeds upwards in ventricle walls in purkyne fibres

73
Q

Why does the cardiac cycle need to be coordinated

A

-heart muscle can initiate its own contraction - myogenic
-the muscle will contract and relax rhythmically even if not connected to body
-muscles from atria and muscles from ventricles each have own natural frequency of contraction
-this property of muscle could cause inefficient pumping if contractions of chambers are not synchronised
-a condition known as fibrillation

74
Q

What are electrocardiograms

A

-we can monitor electrical activity of heart using electrocardiograms
-sensors on skin pick up electrical excitation created by heart and convert this to trace

75
Q

What is haemoglobin

A

-oxygen is transported in RBC
-these cells contain protein haemoglobin
-when oxygen take up by haemoglobin it becomes oxyhaemoglobin
-haemoglobin is complex protein with four subunits
-each subunit consists of polypeptide chain and haem
-haem group contains single iron ion which can attract and hold and oxygen molecule
-haem group has high affinity for oxygen
-each haem group can hold one one oxygen molecule therefore each haemoglobin can carry four oxygen molecules

76
Q

Describe the transport of oxygen

A

-oxygen is absorbed into blood as it passes alveoli in lungs
-oxygen molecules diffuse into plasma and red blood cells - become associated with haemoglobin
-oxygen binds reversibly to haemoglobin - this takes oxygen molecules out of solution and so maintains steep concentration gradient allowing more oxygen to enter blood from lungs and diffuse into cells
-blood carries oxygen from lungs back to heart before travelling around body to supply tissues
-in body tissues, cells need oxygen for aerobic respiration therefore oxyhaemoglobin must be able to release oxygen - dissociation

77
Q

Describe the ability of haemoglobin to transport oxygen

A

-ability of haemoglobin to associate with and release oxygen depends on concentration of oxygen in tissue - the partial pressure of oxygen pO2
-also called oxygen tension kPa
-haemoglobin can associate with oxygen in a way that is produces and s-shaped curve - haemoglobin dissociation curve
-at low oxygen tension haemoglobin does not readily associate with oxygen - this is because haem groups that attract oxygen are in centre of haemoglobin molecules -making them difficult to reach
-this difficulty combining with first oxygen molecule accounts for low saturation level of haemoglobin at low oxygen tensions

78
Q

What happens as partial pressure of oxygen rises

A

-as oxygen tensions rise, diffusion gradient into haemoglobin molecule increases - eventually one oxygen molecule enters the haemoglobin molecules and associates with one of the haem groups
-this causes a slight change in the shape of haemoglobin molecules known as conformational change
-it allows more oxygen molecules to enter haemoglobin molecules and associate with other haem groups relatively easily
-this accounts for steepness of curve as oxygen tension rises

79
Q

What happens near 100% saturation

A

-as haemoglobin approaches 100% saturation, the curve levels off, creating s shape
-mammalian haemoglobin is well adapted to transporting oxygen to tissues of a mamal
-oxygen tension found in lungs is sufficient to produce close to 100% saturation
-this oxygen tension in respiring body tissues is sufficiently low to cause oxygen to dissociate readily from oxyhaemoglobin

80
Q

Describe fetal haemoglobin

A

-fetal haemoglobin has a higher affinity for oxygen
-therefore dissociation curve slightly to left in comparison to adult haemoglobin
-this because fetal haemoglobin will absorb oxygen/ associate where oxygen tension low enough to make adult haemoglobin release oxygen

81
Q

Describe haemoglobin within the placenta

A

-in placenta where oxygen tension is low, fetal haemoglobin will absorb oxygen from surrounding fluid
-this reduces oxygen tension even further
-as a result, oxygen diffuses from mothers blood into placenta
-this reduces oxygen tension within mothers blood which in turn makes maternal haemoglobin release more oxygen

82
Q

How does gene expression play a role in fetal haemoglobin

A

-when baby is born, gene for fetal haemoglobin Hb turns off
-gene for adult Hb turns on
-otherwise the next generation would need even higher affinity for oxygen

83
Q

Describe the chloride shift

A

1) CO2 diffuses from respiring cells and plasma into red blood cells
2) carbon dioxide combines with H2O to form carbonic acid catalysed by enzyme carbonic anhydrase
3)carbonic acid dissociates to release hydrogen ions and hydrogen carbonate ions HCO3-
4) the HCO3- diffuses out of RBC into plasma
5) the charge inside the RBC is maintained by chloride shift (movement of chloride ions from plasma into RBC
6) hydrogen ions are taken up by haemoglobin to produce haemoglobinic acid HHb
7) O2 diffuses out of RBC into plasma

84
Q

Describe the Bohr shift

A

-more respiration =more CO2
-more CO2 present =more O2 released
-muscles need this O2 for aerobic respiration
-therefore more H+ produced
-more H+ ions, lower affinity of haemoglobin
-O2 released at higher PO2
-curve shifts to the right

85
Q

What is the role of haemoglobin

A

-carbon dioxide is released from respiring tissues- it must be removed from these tissues and transported by blood to lungs for excretion
-5% dissolved directly in plasma
-10% combined directly with haemoglobin to form a compound called carbaminohaemoglobin
-85% transported in form of hydrogencarbonate ions HCO3-

86
Q

How does haemoglobin act as a buffer

A

-hydrogen ions building up in RBC could cause contents of RBC to become very acidic
-to prevent this, hydrogen ions are taken out of solution by associating with haemoglobin to produce haemoglobinic acid HHb
-the haemoglobin acts as a buffer to maintain a constant pH

87
Q

What is the effect of increasing carbon dioxide concentration

A

-blood entering respiring tissues carries oxygen as oxyhaemoglobin
-the partial pressure of oxygen in respiring tissues is lower than that in the lungs because oxygen used in respiration
-as result, oxyhaemoglobin begins to dissociate and release oxygen to the tissus
-this means that haemoglobin is available to take up hydrogen ions forming haemoglobinic acid
-where tissues very active there is more carbon dioxide released - this leads to Bohr shift