Transport In Plants Flashcards
Define transpiration
This is the process of water loss in form of water vapour to the atmosphere from the plant.
What are the types of transpiration?
i. Stomatal transpiration
ii. Cuticular transpiration
iii. Lenticular transpiration
Describe the three types of transpiration
Stomatal
- This is the loss of water vapour to the atmosphere through the stomatal pores of the leaves.
- This contributes 90% of the total water loss from a leafy shoot.
- This is because leaves contain a large number of stomata for gaseous exchange where this water vapour can pass and also there’s little resistance to the movement of water vapour through the stomatal pores.
- In addition, leaves also have a large surface area over which water vapour can evaporate rapidly to the atmosphere.
Cuticular transpiration
- This is the loss of water vapour to the atmosphere directly through the epidermis coated with a cuticle layer.
- It contributes 5% to the total water loss from the leafy shoot.
- This is because the cuticle is hard, waxy and less permeable to most diffusing molecules including water vapour molecules.
Lenticular transpiration
- This is the loss of water vapour through a mass of loosely packed cells known as lenticels found scattered on the stems.
- It also contributes 5% of the total water loss to the atmosphere in a leafy shoot.
- It is because the lenticels are usually few in number and not directly exposed to environmental conditions.
- Lenticular transpiration is the main source of water loss from deciduous plants after shading off their leaves. Because there are more stomata on the leaves than elsewhere in the shoot system, it is evidence that most of the water vapour is lost from the leaves.
Describe the structure of a lenticel
- Loosely packed epidermal cells of the stem, non-lignified, capable of losing water by evaporation
What are hydathodes?
Modified stomata found in flowers and leaf margins capable of guttation
What is guttation?
The loss of water from a plant in liquid form.
Describe the structure of a stoma
- A pore, bordered by a pair of bean shaped cells called guard cells that control its opening and closing. The inner layer of the guard cell is thicker and less elastic while the outer is thinner and more elastic.
What assumption is made when using weighing method to determine rate of transpiration?
- Assume mass loss is only due to water loss by transpiration
What assumption is made when using potometer method to determine rate of transpiration?
- Assume water uptake is equal to water loss
What precautions are taken when using a potometer?
- The leafy shoot used should have a significant water loss by having very many leaves
- The stem of the leaf shoot must be cut under water to prevent air from entering and blocking the xylem vessels
- The setup must have plenty of water
- Ensure that only one bubble is present in the capillary tube
- A well graduated scale must be used e.g. a ruler, so that clear readings are taken
- The air bubble should always be reset to zero mark before the potometer is used again under different conditions
- The water reservoir should be filled with water when setting the air bubble at the zero mark
- The cut leafy shoot must be in contact with water in the sealed vessel
What is the ecological significance of transpiration?
- Water moves up a stem, makes it turgid, support the plant parts
- Absorption of water for photosynthesis, primary productivity
What is a criticism of the photosynthetic product theory of stomatal opening and closing?
The theory does not explain how the low rate of glucose formation can account for the rapid opening of stomata
What are the advantages of transpiration?
- It allows the uptake of water from the roots to leaves in form of a transpiration stream. This is due to a transpiration pull created in the leaves. This ensures proper distribution of water throughout the plant to keep it alive.
- It facilitates the uptake of the absorbed mineral salts within the xylem vessels from roots to leaves
- It brings about the cooling of the plant since as water evaporates to the atmosphere, excessive heat is also lost as heat of vaporization, which results into the cooling of the plant
- It brings about mechanical support in non- woody or herbaceous plants, due to water uptake which provides turgidity to the parenchyma cells of the stem and leaves
- It is important for cloud formation via evapotranspiration hence resulting into rainfall
What are the disadvantages of transpiration?
- It causes wilting of plants in case of excessive transpiration
- It may eventually cause death of the plant, when the plant looses water excessively due to excessive transpiration
What are the effects of wilting?
- There is reduction in turgor pressure and drooping of the plant.
- Causes closure of the stomata which cuts off gaseous exchange and therefore may cause death if it persists.
What is the ecological significance of transpiration?
- Plants take up water, plant cells become turgid giving support to plants
- Leaves spread out when cells are turgid and absorb more sunlight
- Absorption of water for photosynthesis which increases primary productivity
What is the physiological significance of transpiration?
- Cools the plant enabling enzyme activity to occur at optimum temperature
What factors affect transpiration?
Environmental:
- Temperature
- Humidity
- Wind/ air currents
- Light intensity
Non-environmental:
- Number of stomata
- Leaf area
- Cuticle
- Leaf arrangement
(Check book for explanations)
What conditions favor stomatal opening?
- High light intensity
- Low mesophyll carbon dioxide levels
Describe the photosynthetic product theory of stomatal openings and closing
- Guard cells have chloroplasts.
- During day light, they carry out photosynthesis producing sugar.
- The sugar lowers the water potential of the cell sap.
- This causes water to move into the guard cells from nighbouring epidermal cells by osmosis.
- The result is an expansion and increase in turgidity of the guard cells containing the stomata to open.
- In darkness, photosynthesis stops and the sugar in the guard cells is converted to starch.
- This raises the water potential of guard cells causing them to lose water to neighboring cells by osmosis.
- The guard cells become flaccid and the stomata close.
Describe the active potassium theory of stomatal opening and closing in plants
In light:
- Starch is converted to malic acid in guard cells
- Malic acid dissociates into malate ions and hydrogen ions
- Blue light activates ATPase and hydrogen ions are actively pumped out of guard cells into neighboring epidermal cells as potassium ions are actively pumped into the guard cells.
- Accumulation of malate ions and potassium ions in the guard cells lowers the water potential of the guard cells.
- However in some plants such as onions where the guard cells have no starch, malate does not accumulate and instead chloride ions are taken up with positive ions to maintain the electrochemical neutrality.
- Water enters into the guard cells by osmosis and guard cells become turgid, stomata open
In darkness:
- Potassium ions diffusion out of the guard cells into neighboring epidermal cells
- Malic acid converts back into starch
- Hydrogen ions re-enter the guard cells via carrier proteins, lowering the pH of guard cells
- Water potential of the guard cell sap increases above that of neighboring epidermal cells
- Water moves out of guard cells into neighboring epidermal cells, they become flaccid and the stomata close
Describe the starch-sugar interconversion theory of stomatal opening and closing
In the day:
- Guard cells photosynthesize
- Decreased concentration of CO2 in guard cells
- Increased pH (alkaline)
- Starch is converted to sugar
- The cell sap concentration increases thus the water potential decreases
- Water enters the guard cells by endosmosis
- Increased turgor in the guard cell
- Stomata open
At night:
- Photosynthesis in guard cells ceases
- Increased concentration of CO2 in guard cells
- Decreased pH (acidic)
- Sugar is converted to starch
- The cell sap concentration decreases thus the water potential increases
- Water leaves the guard cells by exosmosis
- Decreased turgor in the guard cell
- Stomata close
What is the sequence of layers in the root?
Epidermis > Cortex > Endodermis (which has suberin) > xylem vessel
Describe the mechanism of water uptake by plants (ie into the endodermal cells)
Water flows from root hairs endodermal cells using three pathways, namely;
a) Apoplast (cell wall) pathway
b) Symplast (cytoplasm) pathway
c) Vacuolar pathway
Apoplast pathway
- This is the pathway in which water moves through the spaces between the cellulose fibres in the cell wall of one cell to the cell wall of the adjacent cells.
- However, this movement does not occur within the endodermal cells because they possess the impermeable casparian strip which prevents water and solutes flow through the cell walls of the endodermal cells.
- This means that water and solutes flow through the cell walls of the endodermal cells via the Symplast and the vacuolar pathways only.
Symplast pathway
- This is the movement of water through the cytoplasm of one cell to the cytoplasm of the adjacent cell via plasmodesmata.
- Water leaving the pericycle cells to enter the xylem causes the water potential of these cells to become more negative (more dilute).
- This facilitates the flow of water by osmosis from the adjacent cells into these cells.
- In this way the water potential gradient from the root hairs to the xylem is established and maintained across the root.
Vacuolar pathway
- This is the movement of water from the sap vacuole of one cell to the sap vacuole of the adjacent cell following a water potential gradient.
- This is achieved by maintaining a steep water potential gradient.
What adaptations do root hairs in plants have to ensure maximum absorption of water?
a. They are numerous in number so as to provide a large surface area for the maximum absorption of water by osmosis.
b. They are slender and flexible for easy penetration between the soil particles so as to absorb water.
c. The lack a cuticle and this enhances the passive osmotic absorption of water without any resistance
d. They have a thin and permeable membrane which allows the absorption of water by osmosis.
e. They have a water potential lower than that of the soil solution which facilitates a net osmotic flow of water from the soil
By what means does water move up the stem from the roots to the leaves?
- Root pressure
- Transpiration
- Capillarity
Describe the root pressure mechanism
- Root pressure is the force developed by cells of the roots which forces water from the endodermal cells into the xylem vessels of the root and constantly forces water upwards through the stem to leaves.
- This process is active and involves utilization of many ATP molecules.
- Root pressure occurs as a result of endodermal cells actively secreting salts into the xylem sap from their cytoplasm, which greatly lowers the water potential in the xylem.
- In some plants, root pressure maybe large enough to force liquid water through pores called hydathodes of the leaves in a process called guttation
What evidence supports root pressure mechanism of water uptake from the endodermis into the xylem vessel as an active process?
a. There are numerous starch grains in endodermal cells which could act as an energy source for active transport.
b.Lowering the temperature reduces the rate of water exudation (given out) from the cut stem as it prevents root pressure, an active process.
c. Treating the roots with metabolic poisons e.g. potassium cyanide also prevents water from being exuded from the cut stems. This is because the poisons kill the cells thereby preventing aerobic respiration, a source of ATP molecules.
d.Depriving roots of oxygen prevents water from being exuded from the cut stems. This shows that water was being pushed upwards in the cut stem by root pressure, an active pressure.