Translation/Metabolism Flashcards

1
Q

translation substeps

A

Initiation, elongation, and termination

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2
Q

the formation of a 30s and 50s rRNA initiation complex

A

initiation

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3
Q

r-RNA means ribosomal RNA, ____ and ___are the sizes of two parts of the ribosome. If they are joined, they are called ____ ribosome

A

30s and 50s, 70s

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4
Q

All protein synthesis begins with the ____ initiation codon

A

AUG

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5
Q

_____ called initiation factors, and the ___ bond energy from GTP should also be present

A

3 proteins, phosphate

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6
Q

Initial AUG encodes for ____ (special amino acid); middle AUG encodes

A

N-formyl methionine, regular methionine

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7
Q

ten nucleotides upstream the initial AUG

A

Shine-Delgarno box

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8
Q

uses tRNA with attached amino acids as decoders

A

Elongation

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9
Q

match the codons on mRNA

A

anti codons

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10
Q

the mRNA ___ the ribosome then the ribosome reads the message. Upon reading, the ____ produces polypeptides. Once the ___ enters the ribosome, the tRNA helps to produce _____

A

approaches, ribosome, polypeptide

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11
Q

UAA, UAG, and UGA signals to ____. AUG is the ____

A

stop, initiation

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12
Q

Translation start codon

A

AUG

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13
Q

Two sites on the ribosome for the tRNA

A

P and A

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14
Q

the ribosome is moving to balance the elongating polypeptide chain

A

Rachet mechanism

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15
Q

single m-RNA can be read simultaneously by

A

10 to 20

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16
Q

chaperones (proteins) that assist in folding

A

Folding

17
Q

clipped off during secretion

A

signal sequence

18
Q

signal sequence = a sequence of 20-25 amino acids on the front of a protein that directs protein to be secreted out of the cell

A

Secretion

19
Q

secretion through the cytoplasmic membrane

A

Procaryotes

20
Q

bud around the proteins and transport to the membranes

A

transport vesicles

21
Q

Addition of sugars to the protein structure

A

Glycosylation

22
Q

only accomplished in eukaryotic organisms

A

N-linked glycosylation

23
Q

Glycosylation ____ is a target for the body to clear proteins. _____ glycosylation patterns will be ____. Critical implications for producing _____ proteins for human injection.

A

pattern, Non-native, removed, therapeutic

24
Q

the processes by which cells synthesize biomolecules and generate energy

A

Metabolism

25
Q

energy-yielding metabolism.
The process of breaking down larger molecules to extract energy and create reaction substrates

A

Catabolism

26
Q

biosynthetic metabolism, the process of synthesizing larger molecules for maintenance and new cell generation

A

Anabolism

27
Q

waste products from the cell

A

Metabolites

28
Q

utilizable energy that is used in anabolism

A

Adenosine triphosphate (ATP)

29
Q

Levels/Class of Cellular Reactions

A

Class I or Fueling Reactions: the nutrients are absorbed and some of the wastes are removed.
Class II or Biosynthesis Reactions: formed higher or more complicated molecules.
Class III or Polymerization Reactions: biopolymers are formed from monomer units from the Class II.