translation

Question 1

where is the ribosome binding site located?

Answer 1

in the 5’ UTR

Question 2

tRNA structure

Answer 2

3 stem loops, single stranded region (AA attaches), anticodon on loop 2

Question 3

how are tRNAs charged?

Answer 3

enzyme amino-acyl tRNA synthetases

Question 4

where are ribosomal subunits made?

Answer 4

nucleus

Question 5

where are ribosomal subunits assembled?

Answer 5

cytoplasm

Question 6

where is the exit site of the polypeptide

Answer 6

50S subunit

Question 7

what is necessary for initiation?

Answer 7

initiation factors and energy supply from GTP

Question 8

initiation differences in eukaryotes

Answer 8

instead of RBS there is guanosine cap that promotes binding of mRNA and subunit, start codon variable distance from RBS, start tRNA is methionine instead of formyl methionine in bacteria

Question 9

what is the peptidyl transfer reaction?

Answer 9

polypeptide in the P site is transferred to AA in A site by peptidyl transferase

Question 10

what are stop codons recognized by?

Answer 10

release factors