transcription Flashcards
what is transcription?
makes mRNA from the genetic code
what attracts rna polymerase
promotor sequence
what is a promoter structure made of?
-10 (prinbow box) and -35 bases before trc start site
what catalyzes transcription?
RNA polymerase
composition of rna polymerase
multi subunits: b’, b, 2a, omega and sigma
steps of transcription
initiation, elongation, termination
what does the sigma factor of the RNA polymerase do?
scan for promotor sites on DNA
what is a closed promotor complex?
initial binding between promotor site and polymerase when DNA is not unwound
what are transcription factors?
mediate the binding of RNA polymerase forming a transcription initiation complex
how long is the unwound region during elongation
17 base pairs
can RNA polymerase proofread?
yes
types of translation termination
rho dependent and rho independent