Translation Flashcards
1
Q
What is Step 1?
A
Start codon is AUG (Methionine). 40S subunit with Met-tRNA (5’CAU anticodon) along with initiation factors bind at 5’ Cap.
2
Q
What is Step 2?
A
The 40S subunit moves along (ATP Hydrolysis)
3
Q
What is Step 3?
A
The 60S subunit binds to the 40S subunit
4
Q
What is Step 4?
A
P site of Ribosome holds the met-tRNA and the A site holds the next aminoacyl-tRNA which binds to the complementary mRNA codon
5
Q
What is Step 5?
A
A peptide bond forms between amino acids (which are now both held in the A site)
6
Q
What is Step 6?
A
Translocation occurs
7
Q
What is Step 7?
A
Now the A site is empty, aminoacyl-tRNA can bind to the next codon as ribosome moves along. The process repeats.
8
Q
What is the final step?
A
Termination when stop codon is reached and the final tRNA detaches
