Transformation methods for eukaryotes Flashcards

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1
Q

What’s the mechanism behind T-DNA transfer?

A
  1. Plant is recognized if wounded by virA protein in bacteria.
  2. Ti-plasmid is produced (with left- and righ border).
  3. The bacteria transfers all its genetic material from within the left- and right borders into the plant cell.
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2
Q

What’s a floral dip?

A

A floral dip is when you dip an entire plant (including seeds) in an agrobacterium suspension. The seeds become transgenic with whatever genetic material you introduce through T-DNA transfer.

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