Next generation transcriptomics: a practical approach Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

What’s the difference between transcriptomics and exomics?

A

Transcriptomics: All RNA is assayed.

Exomics: All exons are assayed.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Why can’t you find new transcripts using microarrays?

A

The probes which are anchored to each well derive from known sequences.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

In short, describe RNAseq.

A
  1. Purify RNA
  2. Fragment RNA
  3. Generate cDNA
  4. Add adapters to cDNA
  5. Paired-end reads are generated.
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Watch the video on RNAseq.

A

Do it feller.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

When doing RNAseq, what result do you want to attain (raw data)?

A

You want to find the relative molar concentration of RNA in the cell.

In order to find the absolute numbers, you need to add standards.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What does “differential gene expression” mean?

A

The differential gene expression is the change in gene transcription.

False positives are tended to by the boneferoni method: You find the p-value required for any amount of false positives (ex 0.05).

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What’s the gene onthology?

A

The egene ontology (GO) describes our knowledge of a gene with regards to:
1. Function
2. Molecular activity

ex: gene X and Y are grouped together because they both participlate in TCA cycle.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What’s one major benefit of RNAseq compared to microarrays?

A

RNAseq can be used to find novel sequences (you sequence RNA pair-endedly).

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Can you find allele-specific expression using RNAseq?

A

Yes. You sequence all the thangs.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly