PCR and primer design Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

Does the taq polymerase generate any overhang?

A

Yes. 3’ A/AA overhang.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What does the primers’ melting temperature depend on?

A

Ions, solvent, primer length, GC-content.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What affects primer stability and efficiency?

A

The following attributes are positive:
1. Primer 3’ stability
2. No simple regions (repetative)
3. No 3’ complementarity between the primers.
4. Tm for both primers match within 5 degrees.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly