Transformation Flashcards
1
Q
Where is the multiple cloning site?
A
small section on plasmid with restriction enzyme sites
2
Q
Resistance gene
A
AmpR
3
Q
How are cells made competent
A
done at cold temperature with salts like CaCl
4
Q
What happens if cells become warm
A
repair process begins, must remain cold to stop repair
5
Q
what controls are used
A
3 controls
Bacteria w/o antibiotic and no processing
bacteria w/ antibiotic and no processing
Bacteria w/o antibiotic after processing
6
Q
process of transformation
A
heat shock or electroporation
7
Q
process of heat shock
A
Bacteria on ice, moved to 42C for 1 minute, then back to ice
