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Cell Bio Lab > Quantitate DNA > Flashcards

Quantitate DNA Flashcards

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Biology 101 flashcards
1
Q

Absorbance

A

A260/A280= about 1.8 if pure

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2
Q

diluted 1:10 ratio of DNA 100uL

A

10uL of DNA and 90 uL of water

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3
Q

find concentration in DNA

A 
A260 = 1 = 50 ug/mL
A280 = 1.8
Ex. 
A260 = 0.7
0.7 X 50 ug/mL = 35 ug/mL
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Cell Bio Lab (6 decks)

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