Electrophoresis

Question 1

Electrophoresis is:

Answer 1

the separation of charged particles by size in the presence of an electrical current

Question 2

DNA has a ______ charge

Answer 2

negative

Question 3

Used to make the gelatin

Answer 3

1.0% agrose in TBE buffer

Question 4

Dye in gel

Answer 4

ethidium bromide, allows you to visualize the DNA with UV light

Question 5

How are wells created in gel

Answer 5

using a comb while the gel solidifies

Question 6

process of electrophoresis

Answer 6

1. Make gel with comb for wells
2. remove comb
3. put gel in electrophoresis chamber
4. add buffer
5. add loading dye to DNA
6. fill wells
7. run in 120V of current

Question 7

Concentration of loading dye

Answer 7

6X concentration

Question 8

why use loading dye on DNA

Answer 8

makes DNA heavy

has dye that allows you to see how far DNA has moved in gel

Question 9

Wells filled with

Answer 9

10 uL in each well

Question 10

Filled wells

Answer 10

1. 10 uL Plasmid and 2.5 uL loading dye
2. 10 uL PCR
3. Marker ladder
4. 10 uL Plasmid and EcoRI
5. control - 2x buffer, nothing else