Plasmid Isolation Flashcards
1
Q
List of components
A
- Resuspension buffer (RT)
- Lysis Buffer (RT)
- alkaline protease
- neutralization buffer
- DNA binding column
- wash buffer- has ethanol
- nuclease free water
2
Q
resuspension buffer
A
used to resuspend the pellet of cells
3
Q
lysis buffer
A
breaks open cell
contains NAOH, SDS (detergent)
4
Q
alkaline protease
A
breaks down proteins in alkaline condition from NaOH
5
Q
neutralization buffer
A
neutralizes pH with KAc
precipitates protein and genomic DNA
allows the plasmid to remain small and pass through filter
6
Q
DNA binding column
A
catches DNA
7
Q
wash buffer
A
has ethanol, washes protein from filter
8
Q
nuclease free water
A
allow to soak on filter
