Transcriptomics

Question 1

What is Transcriptomics ?

Answer 1

• The study of the transcriptome, the complete set of RNA transcripts that are produced by the genome, under specific circumstances or in a specific cell using high-throughput methods
• provide a broad account of which cellular processes are active and which are dormant

Question 2

what are the 2 key contemporary techniques in transcriptomics ?

Answer 2

• Microarrays – which quantify a set of predetermined sequences
• RNA-Seq – which uses high-throughput sequencing to record all transcripts

Question 3

what are the 2 key contemporary techniques in transcriptomics ?

Answer 3

• Microarrays – which quantify a set of predetermined sequences
• RNA-Seq – which uses high-throughput sequencing to record all transcripts

Question 4

whata re microarrays ?

Answer 4

• Usually consist of a grid of short nucleotide oligomers, known as “probes”, typically arranged on a glass slide
• Transcript abundance is determined by hybridisation of fluorescently labelled transcripts to these probes
• The fluorescence intensity at each probe location on the array indicates the transcript abundance for that probe sequence

Question 4

whata re microarrays ?

Answer 4

• Usually consist of a grid of short nucleotide oligomers, known as “probes”, typically arranged on a glass slide
• Transcript abundance is determined by hybridisation of fluorescently labelled transcripts to these probes
• The fluorescence intensity at each probe location on the array indicates the transcript abundance for that probe sequence

Question 5

how is RNA-seq accomplished ?

Answer 5

• it is accomplished by reverse transcribing RNA in vitro and sequencing the sulting cDNA

Question 6

what is transcript aboundance ?

Answer 6

• The number of counts from each transcript